Title Page
Contents
I. Introduction 8
II. Materials and Methods 11
1. Preparation of extract 11
2. Antioxidant activity 14
2.1. Total phenol content 14
2.2. Total flavonoid content 16
2.3. DPPH radical scavenging activity 18
2.4. ABTS radical scavenging activity 18
2.5. Reducing power 19
2.6. ORAC assay 19
2.7. Statistical analysis 20
3. Anti-inflammatory 20
3.1. Cell culture 20
3.2. Cell proliferation of the RAW 264.7 cell 20
3.3. Nitric oxide (NO) assay 21
III. Results 22
1. Antioxidant activity 22
1.1. Determination of total phenol content 22
1.2. Determination of total flavonoid content 24
1.3. DPPH radical scavenging activity 26
1.4. ABTS radical scavenging activity 28
1.5. Reducing power 30
1.6. ORAC assay 32
2. Anti-inflammatory 35
2.1. Cell proliferation of the RAW 264.7 cell 35
2.2. Nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells 38
IV. Discussion 40
Reference 43
Abstract (국문) 50
ABSTRACT 52
Fig. 1. Dried whole plant of Korean P. urinaria L. 12
Fig. 2. Scheme for the preparation of extract from Korean P. urinaria L. 13
Fig. 3. Calibration curve of gallic acid for total phenol content. 15
Fig. 4. Calibration curve of quercetin for total flavonoid content. 17
Fig. 5. Total phenol content of extract from Korean and Chinese P. urinaria L. Values... 23
Fig. 6. Total flavonoid content of extract from Korean and Chinese P. urinaria L.... 25
Fig. 7. DPPH radical scavenging activity of extract from Korean and Chinese P.... 27
Fig. 8. ABTS radical scavenging activity of extract from Korean and Chinese P.... 29
Fig. 9. Reducing power of extract from Korean and Chinese P. urinaria L. Values are... 31
Fig. 10. Relative fluorescence intensity of extract from Korean and Chinese P. urinaria... 33
Fig. 11. Trolox equivalent content of extract from Korean and Chinese P. urinaria L.... 34
Fig. 12. Cell proliferation of RAW 264.7 cells treated with extract from Korean and... 36
Fig. 13. Photomicrograph of RAW 264.7 cell treated with extract from Korean and... 37
Fig. 14. Inhibition effect of NO production of extract from Korean and Chinese P.... 39