Title Page
Contents
Abstract 7
Introduction 9
Materials and Methods 12
Experimental animals 12
RNA extraction and library construction 13
Analysis of differentially expressed genes (DEGs) 14
Quantitative PCR (qPCR) analysis 14
Results 17
Transcriptomic sequencing and de novo assembly 17
Functional annotation 20
Classification of DEGs induced by ESA 22
Validation of gene expression by qPCR 36
Discussion 38
Conclusion 46
References 48
Table 1. Primer sequences for qPCR validation 15
Table 2. Summary of the transcriptomic data collected from hepatopancreas of L. vannamei 18
Table 3. Metabolism pathways of major DEGs induced by ESA in L. vannamei 28
Table 4. Up-regulated DEGs encoding proteins related to immunity induced by ESA 31
Table 5. Up-regulated DEGs encoding proteins related to cellular transportation induced by ESA 32
Table 6. Up-regulated DEGs encoding proteins related to transcriptional regulation induced by ESA 34
Table 7. Up-regulated DEGs encoding proteins related to molting and maturation induced by ESA 35
Table 8. Comparison of copy numbers between RNAseq and qPCR 37
Supplementary table 1. Up-regulated gene in HP induced by eyestalk ablation. 57
Supplementary table 2. Down-regulated gene in HP induced by eyestalk ablation. 62
Fig. 1. Class distribution of BLASTX top-hits of 105 DEGs. 19
Fig. 2. Second-tier gene ontology (GO) classification of DEGs in response to eyestalk ablation in hepatopancreas of L. vannamei. 21
Fig. 3. Pathways of glycolysis/gluconeogenesis (ko00010). 23
Fig. 4. Pathways of starch and glucose metabolism (ko00500). 24