Title Page
Contents
Abstract 8
1. Introduction 10
2. Materials and Methods 16
2.1. Seaweed and Bacteria 16
2.2. Preparation of Fermented Feed 16
2.3. Extraction of Crude Extract from Ecklonia cava 17
2.4. Visceral Evisceration and Feeding Protocol. 22
2.5. Statistical & Data Analysis 23
2.6. Calculation of Gut Growth 23
2.6.1. Daily Gut Growth 23
2.6.2. Weekly Gut Growth 24
2.6.3. Cumulative Gut Growth 24
2.6.4. Gut Regeneration Efficiency 25
2.7. Preparation of Different Concentrations 25
3. Results 26
3.1. Screening of Crude Extract for bioactivity 26
3.2. Screening for Optimal Concentration of Crude Extract 28
4. Discussion 35
5. Conclusion 41
References 43
Table 1. Average gut growth (mgday -1). Mean values with different superscripts are...(이미지참조) 27
Table 2. Average gut growth rate (mgday-1) for different concentrations. Values are...(이미지참조) 31
Fig. 1. General anatomy of sea cucumber A. japonicus. Adapted from Sun (2010) 13
Fig. 2. Preparation of fermented feed using Undaria pinnatifida seaweed, U53 bacteria and... 18
Fig. 3. Preparation of the fermented seaweed feed. Above (A) is the preparation of the... 19
Fig. 4. Frequency distribution of Undaria pinnatifida seaweed particle sizes after... 20
Fig. 5. Extraction of raw extracts from Ecklonia cava. Modified from Folch et al.(1957) 21
Fig. 6. Above is the original gut at evisceration. Below is the regenerated gut after 27 days'... 30
Fig. 7. Without giving the extract, gut generation is slow. After 27 days, the gut is recovered... 30
Fig. 8. Cumulative weekly gut growths (mg) over a period of four weeks. Superscripts... 32
Fig. 9. Comparing amount of newly regenerated guts relative to their originally eviscerated... 33
Fig. 10. Comparing the gut regeneration efficiency (GRE) in percentage, relative to the... 34