Title Page
Contents
Abstract 9
Chapter 1. Introduction 11
Chapter 2. Materials and Methods 14
2.1. Experimental Design and Diets 14
2.2. Fish and Feeding Trial 16
2.3. Sample Collection and Analysis 17
2.4. Statistical Analysis 19
Chapter 3. Results 20
3.1. Growth Performance 20
3.2. Non-specific Immune Responses 20
3.3. Bacteria Challenge Test 21
3.4. Whole Body Proximate Analysis 21
3.5. Serum Parameters 22
Chapter 4. Discussion and Conclusion 34
References 39
Appendices 8
Appendix 1. Initial data on the feeding trial of Juvenile Amur Catfish fed the experimental diets 47
Appendix 2. 4th week data on the feeding trial of Juvenile amur catfish fed the experimental diets after weighing 48
Appendix 3. Final data on the feeding trial of juvenile amur catfish fed the experimental diets for 8 weeks 49
Appendix 4. Serum parameters of juvenile amur catfish fed the experimental diets for 8 weeks 50
Appendix 5. Cumulative survival of juvenile amur catfish fed the experimental diets after injection with E. tarda 51
Appendix 6. Data on CF, HSI and VSI of juvenile amur catfish fed the experimental diets for 8 weeks 52
Appendix 7. Data on proximate composition of juvenile amur catfish fed the experimental diets for 8 weeks 53
Table 1. Experimental diets and feed additive inclusions used to feed juvenile Amur... 15
Table 2. Growth performance of juvenile Amur catfish fed the experimental diets for 8... 23
Table 3. Non-specific immune responses in juvenile Amur catfish fed the experimental... 28
Table 4. Whole body proximate composition of juvenile Amur catfish fed the... 32
Table 5. Serum parameters in juvenile Amur catfish fed the experimental diets for 8... 33
Fig.1. Weight gain of juvenile Amur catfish fed the experimental diets for 8 weeks. 24
Fig.2. Specific growth rate of juvenile Amur catfish fed the experimental diets for 8... 25
Fig.3. Feed efficiency of juvenile Amur catfish fed the experimental diets for 8 weeks. 26
Fig.4. Protein efficiency ratio of juvenile Amur catfish fed the experimental diets for 8... 27
Fig.5. Lysozyme activity in juvenile Amur catfish fed the experimental diets for 8... 29
Fig.6. Superoxide dismutase inhibition in juvenile Amur catfish fed the experimental... 30
Fig.7. Cumulative survival of juvenile Amur catfish fed the experimental diets after... 31