Title Page
Contents
Abstract 8
Introduction 10
Materials and Methods 14
1. Characteristic of wastewater from the processed squid mantle muscle 14
2. Microorganisms 17
3. Biodegradation of wastewater 17
4. Analytic methods 18
5. Relative protease activity (㎝) 19
6. Thin layer chromatography 19
7. Seed germination test 22
8. Antioxidant activity 24
8.1. DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging activity 24
Results and Discussion 25
1. Biodegradation of wastewater 25
2. Change in protease activity 36
3. Degree of biodegradation 39
4. Germination index (GI) 45
5. Antioxidant activity of biodegraded squid mantle muscle wastewater 48
5.1. DPPH free radical scavenging activity 48
Conclusion 51
References 54
Table 1. Composition of Korean squid 13
Table 2. Rf factors and ninhydrin colours for protein and amino acids 21
Table 3. Composition of original squid-mantle-muscle wastewater 28
Table 4. Amino acids composition resulted from TLC analysis 44
Fig. 1. Characteristic of wastewater from the processed squid mantle muscle. 16
Fig. 2. Changes in pH and ORP during the biodegradation of squid mantle muscle... 29
Fig. 3. Changes in CODCr and TN during the biodegradation of squid mantle muscle...(이미지참조) 34
Fig. 4. Schematic diagram of different N forms present in most surface waters. 35
Fig. 5. Changes in relative protease activity and viable cells during the biodegradation of... 38
Fig. 6. Fractionation of areas of TLC result after UV light exposure. 41
Fig. 7. Relative expression using FIJI software for each fraction of TLC area along the... 42
Fig. 8. Coloration by ninhydrin spray showing the presence of amino acids resulted from... 43
Fig. 9. Results of germination index (GI) for the biodegraded squid-mantle-muscle... 47
Fig. 10. DPPH free radical scavenging activities of the culture supernatants collected... 50