Title Page
Contents
Abstract 9
Introduction 11
Objective 15
Materials and Methods 16
1. Study site 16
1.1. South Jeollanam-do Wando County 16
2. Abalone source 16
3. Acclimation 17
4. Experiment design 17
5. Feed source 18
6. Feeding 20
7. Sample collection 20
8. Histological methods 21
8.1. Dissection 21
8.2. Fixation and Re-fixation 21
8.3. Tissue processing wax 22
8.4. Embedding 22
8.5. Sectioning 22
8.6. Staining 23
8.7. Mounting 24
9. Histological assessment 25
10. Statistical analyse 25
Results 27
1. Evaluation score of juvenile adductor muscle 27
1.1. Evaluation score of juvenile adductor muscle in control group 27
1.2. Evaluation score of juvenile adductor muscle in treatment group 27
2. First sample 30
2.1. Anatomical measurement 30
2.2. Juveniles' adductor muscle histology evaluation 32
3. Second Sample (after one month apply Bio-energy water) 35
3.1. Anatomical measurement 35
3.2. Juveniles' adductor muscle histology evaluation 37
4. Third Sampling (two months of Bio-energy water) 40
4.1. Anatomical measurement (Table 7) 40
4.2. Juveniles' adductor muscle histology evaluation 42
5. Fourth sampling (after 3 months) 45
5.1. Anatomical measurement 45
5.2. Juveniles' adductor muscle histology evaluation 47
Discussion 50
Conclusion 55
Reference 58
Table 1. Nutritional composition of the feed (Woosung Feed Co. Ltd., Korea) 19
Table 2. Score value of adductor muscle histology (Bo Seong et al., 2014) 26
Table 3. Evaluation score of juvenile adductor muscle in control group 28
Table 4. Evaluation score of juvenile adductor muscle in treatment group 29
Table 5. Average, Maximum, and Minimum weight of juvenile abalone in first sample 31
Table 6. Body weight and shell measurement of abalones in second sample 36
Table 7. Body weight and shell measurement of abalones in third sample 41
Table 8. Body weight and shell measurement of abalones in fourth sample 46
Fig. 1. Photos of the adductor muscle in control group before apply bio energy water of... 33
Fig. 2. Photos of the adductor muscle in treatment group before apply bio energy water of... 34
Fig. 3. Photos of 10 individuals of abalone adductor muscle tissue of control group in... 38
Fig. 4. Photos of 10 individual of adductor muscle in treatment group apply one month... 39
Fig. 5. Photos of 10 individuals of adductor muscle abalone in control group after 2... 43
Fig. 6. Photos of 10 individuals of adductor muscle abalone in treatment group after two... 44
Fig. 7. Photos of 10 individual of adductor muscle tissue abalone for three months of... 48
Fig. 8. Photos of 10 individuals of adductor muscle abalone for three months of treatment... 49