Title Page
Contents
Abstract 8
Introduction 9
Materials and Methods 13
Classification and isolation of microalgae 13
Molecular identification of unknown microalgae 14
Scanning electron microscopy morphology analysis 15
Growth rate measurement 16
Results 17
Classification and isolation of microalgae 17
Molecular identification of unknown microalgae 20
Scanning Electron Microscopy Morphology Analysis 24
Growth rate measurement 26
Discussion 30
Conclusion 34
References 35
Appendix 42
Table 1. The list of microalgae isolated from the digestive tract of hard clam 19
Table 2. Cell count at harvest and growth rate of Neorhodella sp. and... 28
Figure 1. Isolated microalgae U4 and U5 18
Figure 2. PCR amplification of the 18S rRNA gene of U4 and U5 20
Figure 3. Comparison of 18S rRNA gene sequences of U4 sample and... 22
Figure 4. Comparison of 18S rRNA gene sequences of U5 sample and... 23
Figure 5. SEM picture of the isolated U4, Neorhodella sp. 24
Figure 6. SEM picture of the isolated U5, Minutocellus sp. 25
Figure 7. Growths of the isolated U4, Neorhodella sp. at three different... 26
Figure 8. Growths of the isolated U5, Minutocellus sp. at three different... 27