Title Page
Contents
Abstract 7
1. Introduction 9
2. Materials and Methods 15
2.1. Experimental animals 15
2.2. Double strand RNA synthesis 17
2.3. Injection of dsRNA and measurement of growth and molting 18
2.4. Total RNA extraction and cDNA synthesis 19
2.5. Full length cDNA cloning 19
2.6. Phylogenetic analysis 20
2.7. End-point RT-PCR 21
2.8. Quantitative Real-time PCR 23
3. Results 25
3.1. Genomic characterization of Liv-adipoR 25
3.2. Expression analysis of Liv-adipoR 29
3.3. Silencing of Liv-adipoR with dsRNA 32
4. Discussion 35
5. Conclusion 42
References 45
Table 1. Primers used for Liv-adipoR 22
Fig. 1. The full-length of Liv-adipoR. 26
Fig. 2. Multiple alignments of Adiponectin Receptors with those from... 27
Fig. 3. Phylogenetic analysis of Liv-AdipoR with other AdipoR family... 28
Fig. 4. Expression level of Liv-adipoR in various tissues of Litopenaeus... 30
Fig. 5. Relative copy number of Liv-AdipoR transcript in Litopenaeus... 31
Fig. 6. Relative copy number of Liv-AdipoR transcript in the feeding... 33
Fig. 7. Relative copy number of Liv-AdipoR transcript in different... 34