Title Page
Contents
Abstract 10
Introduction 12
Materials and methods 17
Seaweed and bacteria 17
Fermentation using microorganism 18
Rearing conditions 20
Experimental setup 20
Feeding 21
Results 22
Fermentation Process 22
Powder Production 22
Reducing Sugars 23
Viscosity 24
Change of bacterial number 25
Change in alginate content 26
Calculation of growth performance parameters 27
Growth Performance of Undaria pinnatifida (U) and Undaria pinnatifida fermented by 52 bacteria (U52) 28
Weight gain (WG %) and specific growth rate (SGR) 28
Ingestion rate (IR) and feed conversion ratio (FCR) 30
Growth Performance of Undaria pinnatifida (U) and Undaria pinnatifida fermented by 53 bacteria (U53) 32
Weight gain (WG %) and specific growth rate (SGR) 32
Ingestion rate (IR) and feed conversion ratio (FCR) 34
Average body weight 36
Growth performance of Laminaria Japonica and Laminaria japonica fermented by GR2 bacteria. 38
Weight gain (WG %) and specific growth rate (SGR) 38
Ingestion rate (IR) and feed conversion ratio (FCR) 40
Average body weight 42
Discussion 44
Conclusion 48
References 49
Fig.1. Seaweed fermentation method. 19
Fig.2. Procedures making feeds and feeding. 21
Fig.3. Change in the amount of powder production during fermentation. 22
Fig.4. Change in amount of reducing sugar during fermentation. 23
Fig.5. Change in viscosity during fermentation. 24
Fig.6. Change bacteria number during fermentation. 25
Fig.7. Change in alginate content during fermentation. 26
Fig.8. Weight gain of sea cucumbers fed fresh and fermented Undaria... 29
Fig.9. Specific growth rate (SGR) of sea cucumbers fed fresh and fermented... 29
Fig.10. Ingestion rate (IR) of sea cucumbers fed fresh and fermented... 31
Fig.11. Feed conversion ratio (FCR) of sea cucumbers fed fresh and... 31
Fig.12. Weight gain of sea cucumbers fed fresh and fermented Undaria... 33
Fig.13. Specific growth rate (SGR) of sea cucumbers fed fresh and... 33
Fig.14. Ingestion rate (IR) of sea cucumbers fed fresh and fermented... 35
Fig.15. Feed conversion ratio (FCR) of sea cucumbers fed fresh and... 35
Fig.16. Average body weight of sea cucumbers fed fresh and fermented... 37
Fig.17. Weight gain (WG %) of sea cucumbers fed fresh and fermented... 39
Fig.18. Specific growth rate (SGR) of sea cucumbers fed fresh (L) and... 39
Fig.19. Ingestion rate (IR) of sea cucumbers fed fresh and fermented... 41
Fig.20. Feed conversion ratio (FCR) of sea cucumbers fed fresh and... 41
Fig.21. Average body weight of sea cucumbers fed fresh Laminaria... 43