표제지
목차
Abstract 9
I. 서론 10
II. 재료 및 방법 12
1. 재료 12
2. 실험 방법 12
2-1. 일반성분 분석 12
2-2. 해삼 효소 가수분해물의 제조 12
2-3. 아미노산 분석 14
2-4. 아미노질소 분석 14
2-5. 콘드로이틴황산 분석 15
2-6. 평균 분자량 분석 16
2-7. 효소 가수분해 최적화 실험 설계 및 자료 분석 16
III. 결과 및 고찰 20
1. 일반성분 20
2. 해삼 효소 가수분해물의 특성 20
2-1. 유리 아미노산 함량의 변화 20
2-2. 아미노질소 함량의 변화 24
2-3. 콘드로이틴황산 함량의 변화 27
2-4. 평균 분자량의 변화 29
3. 효소 가수분해 최적화 설계 및 자료 분석 29
3-1. 통계분석 29
3-2. 반응표면 설계 34
3-3. 가수분해 최적화 43
IV. 요약 49
참고문헌 50
Table 1. Enzymes used to hydrolyze sea cucumber 13
Table 2. Central composite design in terms of response surface... 17
Table 3. Programming central composite design for response... 19
Table 4. Proximate composition of sea cucumber 21
Table 5. Total amino acid contents of sea cucumber 22
Table 6. Free amino acid contents of sea cucumber hydrolysate by a... 23
Table 7. Free amino acid contents of sea cucumber hydrolysate by a... 25
Table 8. Amino nitrogen contents of sea cucumber hydrolysate by a... 26
Table 9. Amino nitrogen contents of sea cucumber hydrolysate by a... 28
Table 10. Chondroitin sulfate contents of sea cucumber hydrolysate by a... 28
Table 11. Chondroitin sulfate contents of sea cucumber hydrolysate by a... 28
Table 12. Average molecular weight of sea cucumber hydrolysate by... 30
Table 13. Average molecular weight of sea cucumber hydrolysate by a... 32
Table 14. Analysis of amino nitrogen (Y₁) and chondroitin sulfate... 35
Table 15. Estimated regression coefficient for the amino nitrogen... 36
Table 16. Estimated variance analysis of amino nitrogen contents... 37
Table 17. Estimated regression coefficient for chondroitin sulfate... 39
Table 18 . Estimated variance analysis of chondroitin sulfate... 40
Fig. 1. Gel permeation chromatography analyzer (PL-GPC220). 17
Fig. 2. Average molecular weight of sea cucumber hydrolysate... 31
Fig. 3. Average molecular weight of sea cucumber hydrolysate... 33
Fig. 4. Contour plot of amino nitrogen content (Y₁) vs enzyme... 41
Fig. 5. Surface plot of amino nitrogen content (Y₁) vs enzyme... 42
Fig. 6. Contour plot of chondroitin sulfate content (Y₂) vs enzyme... 44
Fig. 7. Surface plot of chondroitin sulfate content (Y₂) vs enzyme... 45
Fig. 8. Optimization of amino nitrogen content (Y₁) on enzyme... 46
Fig. 9. Optimization of chondroitin sulfate content (Y₂) on enzyme... 48