표제지
목차
I. 서론 9
II. 재료 및 방법 12
1. 실험재료 12
1) 시료 12
2) 시료 추출 및 분획 12
3) 시약 및 기기 15
2. 실험 방법 17
1) 총 폴리페놀 함량 측정 17
2) 항산화 효과 측정 18
3) 항염증 효과 측정 22
4) 백급 분획물을 함유한 에멀젼 제조 28
5) 백급 분획물을 함유한 에멀젼의 안정성 측정 31
6) 백급 분획물을 함유한 에멀젼의 안전성 측정 35
7) 통계처리 36
III. 결과 및 고찰 37
1. 백급 추출물의 수율 37
2. 총 폴리페놀 함량 38
3. 항산화능 측정 결과 39
1) 전자공여능 확인 39
2) ABTS radical cation decolorization 활성 41
3) Superoxide dismutase(SOD) 유사활성 확인 43
4) Hydrogen peroxide(H₂O₂) 라디칼 저해활성 확인 46
4. 백급의 분획 및 수율 48
5. 분획물의 총 폴리페놀 함량 49
6. 분획물의 항산화 효과 50
1) 전자공여능 확인 50
2) ABTS 라디칼 저해활성 확인 52
3) Superoxide dismutase(SOD) 유사활성 확인 54
4) Hydrogen peroxide(H₂O₂) 라디칼 저해활성 확인 56
7. 분획물의 항염증 효과 58
1) Mouse macrophage cell(RAW 264.7)의 세포생존율 58
2) Nitirite 소거활성 59
3) Western blot을 통한 iNOS,COX-2 단백질의 발현 측정 61
4) Human keratinocyte cell(HaCaT)의 세포생존율 63
5) TARC/CCL17 chemokine 발현 측정 65
8. 백급 분획물을 함유한 에멀젼의 안정성 확인 67
1) pH 측정 67
2) 점도 측정 69
3) 온도에 따른 안정성 시험 71
4) 온도순환(Cycle chamber)에 따른 특수·가혹 보존 시험 72
5) 광안정성 시험 73
9. 백급 분획물을 함유한 에멀젼의 안전성 확인 75
1) 첩포 실험 75
IV. 결론 76
V. 참고문헌 79
초록 85
Abstract 87
Table 1. Formulations of emulsion contain EtOAc layer isolated from MB. 29
Table 2. Check points in stability test of emulsion containing in EtOAc layer isolated from MB constant temperature condition 32
Table 3. Reaction of patch test suggested by International Contact Dermatitis Research Group 35
Table 4. Yields of BS extracts 37
Table 5. The contents of total polyphenol from BS extracts 38
Table 6. Yields of MB fractions 48
Table 7. The contents of total polyphenol from MB fractions 49
Table 8. Results of stability test of emulsion containing EMB in constant temperature conditions. 71
Table 9. Stability test of emulsion containing EMB in cycling temperature chamber condition with 1 cycle for 5days. 72
Table 10. Results of artificial sun lamp test of emulsion containing EMB. 73
Table 11. Results of sun test of emulsion containing EMB. 74
Table 12. Results of patch test on emulsion containing EMB. 75
Fig. 1. Procedure for extraction of BS. 13
Fig. 2. Procedure for fraction of Methanol-extracts from BS. 14
Fig. 3. Preparation of emulsion containing EtOAc layer isolated from MB. 30
Fig. 4. Cycle temperature change with day for 1 cycle. 33
Fig. 5. Electron donating ability of BS extracts. 40
Fig. 6. ABTS radical cation scavenging activity of BS extracts 42
Fig. 7. Superoxide dismutase (SOD)-like activity of BS extracts 45
Fig. 8. Hydrogen peroxide scavenging activity of BS extracts 47
Fig. 9. Electron donating ability of fraction of MB fractions 51
Fig. 10. ABTS radical cation scavenging activity of MB fractions 53
Fig. 11. Superoxide dismutase (SOD)-like activity of MB fractions 55
Fig. 12. Hydrogen peroxide scavenging activity of MB fraction 57
Fig. 13. Cell viability rate of EMB on RAW 264.7 cell 58
Fig. 14. The effects of EMB on NO production in RAW 264.7 cells. 60
Fig. 15. The effects of EMB on iNOS and COX-2 protein expression in RAW 264.7 cells. 62
Fig. 16. Cell viability rate of EMB on HaCaT cell. 64
Fig. 17. The effects of EMB on TNF-α and IFN-γ induced TARC/CCL17 expression in HaCaT cells. 66
Fig. 18. pH measurement of the lotion containing EMB 68
Fig. 19. Viscosity measurement of the lotion containing EMB. 70