Title Page
Contents
Introduction 7
Materials and methods 10
1) Plant materials and DNA extraction 10
2) RAPD analysis 12
3) Amplification of the ITS region and purification of PCR products 12
4) Cloning and sequencing of ITS region 13
5) Phylogenetic analysis of the ITS sequences 13
Results and Discussion 15
1) RAPD analysis 15
2) ITS sequence analysis 22
References 30
국문초록 34
ABSTRACT 36
Table 1. Collection areas for seven species of Theaceae used in this study 11
Table 2. The selected five primers, their sequence information, and the application results on seven samples of Theaceae. 15
Table 3. Genetic dissimilarity matrix calculated by Nei(1979)' s genetic coefficient based on RAPD's analysis of Theaceae 20
Table 4. Sequence characteristics of ITS 1 and ITS 2 regions in 7 species of Theaceae 26
Table 5. Sequence divergences in the ITS regions of Theaecae. of the diagonal are divergence values corrected for multiple substitutions using the two parameter model 27
Figure 1. RAPD amplification patterns of Theaceae with OPA 06 16
Figure 2. RAPD amplification patterns of Theaceae with OPA 08 16
Figure 3. RAPD amplification patterns of Theaceae with OPA 09 17
Figure 4. RAPD amplification patterns of Theaceae with OPA 10 17
Figure 5. RAPD amplification patterns of Theaceae with OPA 11 18
Figure 6. UPGMA dendrogram of Theaceae based on a distance matrix generated by method of Nei & Li(1979) 21
Figure 7. PCR products amplified from seven species of Theaceae with ITS-1 and ITS-4 primers. 23
Figure 8. DNA alignment of seven species in Theaceae obtained from ITS 1 and 2 sequencing analysis 25
Figure 9. Phylogenetic tree of seven species in Theaceae according to observed divergence distance method analyzed by DNAMAN 6.0 software. 28