Title Page
Contents
ABSTRACT 7
I. INTRODUCTION 9
II. MATERIALS AND METHODS 12
1. Tissue preparation 12
2. Measurements of muscle tension 12
3. Fura-2 loading and simultaneous measurements of tension and [Ca2+]i(이미지참조) 13
4. Permeabilized preparation 14
5. Data analysis 15
III. RESULTS 16
1. Relaxing activity of Y-27632 16
2. Effects of L-NAME and ODQ on Y-27632-induced relaxation 16
3. Effects of Y-27632 on carbachol-induced contractions 16
4. [Ca2+]i and tension changes produced by high K+ and carbachol(이미지참조) 20
5. Effects of Y-27632 on carbachol-induced increases in [Ca2+]i and tension(이미지참조) 20
6. Effects of Y-27632 on Ca2+-induced contraction in permeabilized muscle(이미지참조) 23
IV. DISCUSSION 25
V. CONCLUSION 30
REFERENCES 31
ABSTRACT(Korean) 35
Fig. 1. Concentration-response curves for the relaxant effect of Y-27632 on contraction induced by 60 mM KCl and 3 μM carbachol. 17
Fig. 2. Effects of L-NAME and ODQ on the concentration-response curves to Y-27632. 18
Fig. 3. Concentration-response curves to carbachol in the absence and presence of Y-27632. 19
Fig. 4. A, Changes in [Ca2+]i and tension produced by depolarization with 60 mM KCl and receptor activation with carbachol.(이미지참조) 21
Fig. 5. A, Effects of Y-27632 on carbachol-induced [Ca2+]i (upper trace) and muscle tension (lower trace).(이미지참조) 22
Fig. 6. A, Effect of Y-27632 on the contraction evoked by Ca2+ in α-toxin-permeabilized esophagus smooth muscle.(이미지참조) 24