Title Page
Contents
ABSTRACT 7
I. INTRODUCTION 9
II. MATERIALS AND METHODS 11
1. Tissue preparation 11
2. Measurements of muscle tension 11
3. Data analysis 12
III. RESULTS 13
1. Effects of testosterone and DHT on KCI- or histamine-induced contractions 13
2. Effects of nitric oxide on testosterone-induced relaxation 13
3. Effects of blockade of androgenic receptor, aromatase and 5a-reductase on testosterone-induced relaxation 17
4. Effects of protein kinase inhibitors on testosterone-induced relaxation 17
5. Effects of K+ channel blockers on testosterone-induced relaxation(이미지참조) 17
6. Effects of testosterone on Ca2+-induced contraction(이미지참조) 18
IV. DISCUSSION 23
V. CONCLUSION 28
REFERENCES 29
ABSTRACT(Korean) 33
Fig. 1. Average concentration-response curves for the relaxant effect of testosterone on contractions induced by 50 mM KCI and 3 μM histamine. 14
Fig. 2. Average concentration-response curves for the relaxant effect of testosterone and DHT in preparations precontracted with 3 μM histamine. 15
Fig. 3. Effects of L-NAME, ODQ and epithelium removal on the average concentration-response curves to testosterone. 16
Fig. 4. Effects of flutamide, aminoglutethimide and finasteride on the average concentration-response curves to testosterone. 19
Fig. 5. Effects of PKA-IM, KT5823 and calphostin C on the average concentration-response curves to testosterone. 20
Fig. 6. Effects of glibenclamide, iberiotoxin and 4-AP on the average concentration-response curves to testosterone. 21
Fig. 7. Concentration-response curves to extracellular calcium (CaCl2) in the absence and in the presence of testosterone (0.1 and 1 μM). 22