표제지
목차
ABSTRACT 9
I. 서론 11
1. 연구의 배경 11
2. 연구의 목적 및 범위 16
II. 이론적 배경 17
1. 피부의 구조 17
1.1. 표피 19
1.2. 진피 20
1.3. 피하지방 21
2. 여드름의 형태 및 구조 22
2.1. 피지의 과잉분비-여드름 초기 (micro-comedo) 22
2.2. 흰 여드름,검은 여드름-가벼운 면포 (comedo) 22
2.3. 붉은 여드름-구진성 여드름 (papule) 23
2.4. 화농성 여드름-농포 (pustule), 결절 (nodule), 낭포 (cyst) 23
3. 여드름의 발생 요인 25
4. 여드름의 악화 요인 27
5. 모발의 구조 및 특성 29
5.1. 모발의 구조 29
5.2. 모발의 특성 32
5.3. 모발의 손상요인 33
III. 연구재료 및 실험방법 34
1. 실험재료 및 장비 34
2. 동백 농축액 제조 34
3. 동백씨 오일의 제조 36
4. 동백잎 추출물의 총 flavonoids 및 phenolics 화합물 함량 분석 37
4.1. Flavonol 화합물 및 catechin 류 화합물 분석 37
4.2. 동백잎 추출물의 항산화 실험방법 41
5. 항균실험 추출물 및 분획물의 조제 43
5.1. 균주의 배양조건 43
5.2. P. acnes의 생육저해율 측정 (paper disc approach) 45
5.3. P. acnes의 항균정량 실험 45
6. 모발실험 제작 46
6.1. 실험모발 46
6.2. 퍼머넌트제의 성분 46
7. 화장품 제형의 제조 48
8. 임상실험 49
IV. 결과 및 고찰 50
1. Hair 화장료 효과 50
2. 피부 효과 (여드름 효과) 59
3. 항산화 효과 61
4. 항산화 성분 70
4.1. Flavonoids (플라보이드) 70
4.2. Polyphenol (폴리페놀) 77
5. 항균실험 결과 85
V. 결론 86
참고문헌 87
Table 1. HPLC method for analysis of flavonoids 38
Table 2. HPLC method for analysis of catechins 39
Table 3. Media compositions for P. acnes (200 basis) 44
Table 4. DPPH radical scavenging effect (%) of Camellia japonica L. 68
Table 5. OH radical scavenging effect (%) of Camllia japonica L. 68
Table 6. Flavonoids of Camellia japonica L. extract (water layer) 76
Table 7. Polyphenols of Camellia japonica L. extract (water layer) 83
Fig.1. Structure of hair root. 18
Fig.2. Structure of hair shafts. 31
Fig.3. Schematic diagram of Camellia japonica L. partitioning composed of... 35
Fig.4. Refining process of camellia oil 36
Fig.5. Condition of radical measurment in EPR spectrometer. 42
Fig.6. Photographs of prepared hair essence products, simple base formulation (a),... 48
Fig.7. SEM images for morphological change of hair cuticle by treatment. 51
Fig.8. SEM images for morphological change of hair cuticle by treatment. 52
Fig.9. SEM images for morphological change of hair cuticle by treatment. 53
Fig.10. SEM images for morphological change of hair cuticle by treatment. 54
Fig.11. SEM images for morphological change of hair cuticle by treatment. 55
Fig.12. SEM images for morphological change of hair cuticle by treatment. 56
Fig.13. Clinical trials of patients by skin tonner C. japonica L. extract 59
Fig.14. DPPH radical scavaagiug effect (heat-treated Camella japonica L. tea) 62
Fig.15. DPPH radical scavenging effect (steamed Camella japonica L. tea) 63
Fig.16. DPPH radical scavenging effect (fermented Camella japonica L. tea) 64
Fig.17. OH radical seavenging effect (heat-treated Camella japonica L. tea) 65
Fig.18. OH radical scavenging effect (steamed Camella japonica L. tea) 66
Fig.19. OH radical scavanging effect (fermented Camella japonica L. tea) 67
Fig.20. Chemical structure of flavonoids in this study. 72
Fig.21. Calibration curve of naringin for analysis of total flavonoids. 73
Fig.22. Chromatogram results of retention time (RT) of myricetin/ quercetin/ kampferol in HPLC. 73
Fig.23. Standard curves of myricetin (a)/ quercetin (b)/ kampferol (c) in HPLC. 74
Fig.24. Chromatogram of flavonoids of C. Japonica L. extract (water layer) in HPLC. 75
Fig.25. Calibration curve of gallic acid for analysis of total phenolic compounds. 78
Fig.26. Chromatogram results of retention time (RT) of EGC/ C/ EC/ EGCG/ GCG/ ECG... 79
Fig.27. Standard curves of ECG (a)/ C (b)/ EC (c)/ EGCG (d)/ GCG (e)/ ECG (f) in HPLC 81
Fig.28. Detailed chromatograms of polyphenolic compounds of Camellia japonica L. extract... 82
Fig.29. Antimicrobial activity of C. japonica L. extracts against p. acnes 85