표제지
목차
I. 서론 8
II. 재료 및 방법 13
1. 실험 재료 13
2. 실험 방법 13
(1) 굴중성 반응과 길이 생장 측정 13
(2) 에틸렌 생성량 측정 14
(3) in vitro ACC synthase (ACS) 활성 측정 14
(4) in vitro ACC oxidase (ACO) 활성 측정 15
(5) 유전자 발현 분석 15
(6) 데이터 분석 18
III. 결과 및 고찰 19
1. 광원에 따른 애기장대 phytochrome mutants 줄기의 생장 반응 19
2. 광원에 따른 애기장대 phytochrome mutants 줄기의 굴중성 반응 25
3. 광원에 따른 애기장대 phytochrome mutants 줄기의 에틸렌 생합성 31
4. 광원에 따른 애기장대 phytochrome mutants 줄기의 ACS와 ACO의 활성 34
5. 광원에 따른 애기장대 phytochrome mutants 줄기의 ACS와 ACO의 유전자 발현 38
IV. 적요 42
V. 참고 문헌 44
Abstract 50
Table1. Sequences of oligonucleotide primers used for qReal-time PCR 17
Figure 1. Ethylene biosynthesis pathway in higher plants 12
Figure 2. Shoot elongation in phytochrome mutants of Arabidopsis... 21
Figure 3. Shoot elongation in phytochrome mutants of Arabidopsis... 22
Figure 4. Shoot elongation in phytochrome mutants of Arabidopsis... 23
Figure 5. Shoot elongation in phytochrome mutants of Arabidopsis... 24
Figure 6. The kinetics of shoot gravitropic response in phytochrome... 27
Figure 7. The kinetics of shoot gravitropic response in phytochrome... 28
Figure 8. The kinetics of shoot gravitropic response in phytochrome... 29
Figure 9. The kinetics of shoot gravitropic response in phytochrome... 30
Figure 10. Ethylene production in phytochrome mutants of Arabidopsis... 33
Figure 11. In vitro ACS activity in phytochrome mutants of Arabidopsis... 36
Figure 12. In vitro ACO activity in phytochrome mutants of Arabidopsis... 37
Figure 13. Relative transcript level of ACS12 gene in phytochrome... 40
Figure 14. Relative transcript level of ACO2 gene in phytochrome... 41