표제지
목차
I. 서론 8
1. 현대사회의 식생활 문화 8
2. 활성산소 8
3. 항산화제의 이용 9
4. 천연항산화제의 연구 사례 10
5. 초석잠의 활용 10
II. 재료 및 방법 13
1. 추출물의 준비 13
2. 항산화능 측정 13
1) DPPH radical scavenging activity 13
2) ABST+ radical scavenging activity(이미지참조) 14
3) FRAP activity 15
3. 성분분석 15
1) Total phenolic content 15
2) Flavonoid content 15
4. 아질산 소거능 평가 16
5. 항균활성능 조사 16
6. 기능성 즉석식품 연구 17
1) 시료 준비 17
2) pH 18
3) 육색 18
4) Cooking loss 18
5) 지방산패도(thiobarbituric acid reactive substances, TBARS) 19
6) 단백질 변패도(volatile basic nitrogen, VBN) 19
7) 미생물학적 특성 평가 20
8) 관능특성 평가 20
7. 통계처리 21
III. 결과 및 고찰 22
1. 초석잠 추출물의 항산화능 22
1) DPPH radical scavenging activity 22
2) ABST+ radical scavening activity(이미지참조) 23
3) FRAP activity 24
2. 성분분석 26
1) Total phenolic and flavonoid content 26
3. 아질산 소거능 28
4. 항균 활성 30
5. 초석잠 추출물이 첨가된 기능성 즉석식품의 품질 특성 35
1) pH 35
2) 육색 37
3) TBARS과 VBN 39
6. 미생물 42
7. 관능적 특성 43
IV. 결론 45
V. 참고문헌 47
국문초록 56
Abstract 58
Table 1. Total phenolic and flavonoid content of Stachy sieboldii MIQ extracts at aconcentration of 100 ug/mL 26
Table 2. Antimicobial activity of Stachy sieboldii MIQ extracts against 8 microorganisms at each concentration (4, 2, 1 and 0.2 mg, dry base) 30
Table 3. pH of fresh pork patties added with Stachy sieboldii MIQ methanol extracts during storage at 4℃. 35
Table 4. Meat color of fresh pork patties added with Stachy sieboldii MIQ methanol extracts during storage at 4℃. 38
Table 5. TBARS of fresh pork patties added with Stachy sieboldii MIQ methanol extracts during storage at 4℃. 39
Table 6. VBN of fresh pork patties added with Stachy sieboldii MIQ methanol extracts during storage at 4℃. 40
Table 7. TPC, coliform, macconkey agar, mannitol salt agar. listeria agar counts of patties with different additives Stachy sieboldii MIQ during storage at 4℃. 42
Table 8. Sensory evaluation of fresh pork patties added with Stachy sieboldii MIQ methanol extracts during storage at 4℃. 43
Fig. 1. Figure of Stachy sieboldii MIQ. 11
Fig. 2. Growth of Stachy sieboldii MIQ. 12
Fig. 3. DPPH radical scavenging activities of Stachy sieboldii MIQ extracts at a concentration of 100ug/mL. 22
Fig. 4. ABTS+ radical scavenging activities of Stachy sieboldii MIQ extracts at a concentration of 500ug/mL.(이미지참조) 23
Fig. 5. Ferric reducing ability of power activities of Stachy sieboldii MIQ extracts at a concentration of 500ug/mL. 25
Fig. 6. Nitrite scavenging activities of Stachy sieboldii MIQ extracts at a concentration of 500 ug/mL. 28
Fig. 7. Inhibitory effect of Stachy sieboldii MIQ methanol extract on the growth of Salmonella typhimurium. 32
Fig. 8. Inhibitory effect of Stachy sieboldii MIQ methanol extract on the growth of Listeria monocytogenes 33
Fig. 9. Inhibitory effect of Stachy sieboldii MIQ methanol extract on the growth of Bacillus cereus 34