표제지
국문초록
목차
I. 서론 12
II. 이론적 배경 15
1. Biofuel cell 15
2. Biofuel cell의 특징 19
3. Biofuel cell의 작동원리 20
4. Mediator 22
1) Direct Electron Transfer 22
2) Mediated Electron Transfer 22
5. Biofuel cell의 응용 25
III. 실험 장치 및 방법 28
1. 시약 28
2. 전극의 제조 29
1) 전극 세척 29
2) Glassy carbon(GC) 전극 표면의 성능 test 29
3) Chitosan membrane 방법 29
4) Sol-gel Matrix 방법 30
5) 연료전지 특성 30
3. 분석기기 32
IV. 결과 및 고찰 34
1. 전극 표면의 성능 test 34
2. GOx 고정화 전극 38
3. Chitosan membrane법 40
1) Fc/GOx/CHI 전극 40
2) CNT/Fc/GOx/CHI 전극 44
4. Sol-gel matrix법 48
1) Fc/GOx/sol-gel 전극 48
2) CNT/Fc/GOx/sol-gel 전극 52
5. 연료전지 특성 55
V. 결론 57
VI. 참고문헌 59
Abstract 62
Table 1. Inorganic fuel cell properties 18
Table 2. The potential application of biofuel cell 27
Figure 1. Schematic of a biofuel cell (a) and electron transfer process from enzyme to electrode (b). 21
Figure 2. Alternative electron-transfer mechanisms. Direct electron transfer (tunneling mechanism) from electrode surface to the active site of an enzyme (a). Electron transfer via redox mediator (b). 24
Figure 3. A typical cyclic voltammogram with cyclic potential wave form (a) and a linear sweep voltammogram with linear sweep potential wave form (b), Three electrode cell system (c) 33
Figure 4. CV cycling of GC electrodes in 1 mM ferricyanide solution; scan rate 50 ㎷/s; cycle# 1 to 5, Reference. 36
Figure 5. CV cycling of two GC electrodes, used in this study, in 1 mM ferricyanide solution. 37
Figure 6. Cyclic voltammograms of GOx-immobilized glassy carbon electrode in 0 mM, 40 mM and 80 mM glucose in pH 7 phosphate buffer.... 39
Figure 7. Cyclic voltammograms of ferrocene and GOx-immobilized glassy carbon electrode using chitosan membrane in pH 7 phosphate buffer contained 0~100 mM glucose (a) and anodic peak... 42
Figure 8. Cyclic voltammograms of ferrocene and GOx-immobilized glassy carbon electrode using chitosan membrane in pH 7 phosphate buffer with changing scan rate (0~200 ㎷/s) (a) and... 43
Figure 9. Cyclic voltammograms of CNT/ferrocene/GOx-entrapped electrode by chitosanin 0~100 mM glucose (a) and changing anodic peak current vs. glucose concentration 0~100 mM (b).... 46
Figure 10. Cyclic voltammograms of CNT/ferrocene/GOx-entrapped electrode by chitosan in pH 7 buffer with changing scan rate 1~200 ㎷/s (a) and anodic and cathodic peak current vs. scan... 47
Figure 11. Cyclic voltammograms of ferrocene and GOx-entrapped electrode by sol-gel in 0~100 mM glucose (a) and changing anodic peak current vs. glucose concentration 0~100 mM (b).... 50
Figure 12. Cyclic voltammograms of ferrocene and GOx-entrapped electrode by sol-gel in pH 7 buffer with changing scan rate (1~200 ㎷/s) (a) and anodic and cathodic peak current vs. root scan rate... 51
Figure 13. Cyclic voltammograms of sol-gel electrode contained CNT in 0~100 mM glucose (a) and changing anodic peak current vs. glucose concentration 0~100 mM (a). 53
Figure 14. Cyclic voltammograms of sol-gel electrode contained CNT in pH 7 buffer with changing scan rate (1~200 ㎷/s) (a) and anodic and cathodic peak current vs. scan rate (b). 54
Figure 15. Complete fuel cell performance of CNT/Fc/GOx-based anode and Fc/BOD-based cathode. Anode was entrapped with chitosan (a) or sol-gel matrix (b). 56