표제지
국문초록
Abbreviation
목차
I. 서론 11
II. 실험방법 14
1. 재료 14
1) 소목 추출 및 분리 14
2) 화합물 분리 동정 및 시료 제조 14
3) 세포 14
2. 방법 14
1) RAW 264.7 Cell 배양 14
2) 파골세포 생성 측정 15
3) 파골세포 유전자발현 측정 15
4) Western Blotting 18
3. 통계학적 분석 18
III. 실험결과 20
1. 파골세포 분화에 미치는 영향 20
2. TRAP, NFATc1, MITF 발현에 미치는 영향 23
3. iNOS, COX-2, TNF-α 및 IL-6 발현에 미치는 영향 26
4. MMP-9 발현에 미치는 영향 29
5. Cathepsin K 발현에 미치는 영향 31
6. MAPK 활성에 미치는 영향 33
7. 핵자기공명분광법 (NMR) 및 질량분광법 (MS)에 의한 brazilin 구조의 분석 35
IV. 고찰 44
V. 결론 49
VI. 참고문헌 50
Abstract 58
Table I. Primers 19
Table II. Effects of Brazilin on the expression of TRAP, NFATc1, MITF in RANKL stimulated RAW 264.7 cells. 25
Table III. Effects of Brazilin on sRANKL induced iNOS, COX-2 ,IL-6 and TNF-alpha mRNA expression level in RAW 264.7 cells. 28
Table IV. ¹H- and ¹³C-NMR spectral data of brazilin 40
Fig. 1. Schematic depiction of the RANKL-RANK signaling pathway in Osteoclast 12
Fig. 2. Chemical Structure of Brazilin 13
Fig. 3. Effects of brazilin on osteoclast differentiation in RANKL stimulated RAW 264.7 cell. 21
Fig. 4. Effects of Brazilin on the Formation of TRAP(+) MNCs in RANKL stimulated RAW 264.7 cells. 22
Fig. 5. Effects of Brazilin on mRNA expression of osteoclast-specific genes in RANKL induced RAW 264.7 cells. 24
Fig. 6. Effects of Brazilin on the expression of MMP-9 in RANKL stimulated RAW 264.7 cells. 30
Fig. 7. Effects of Brx on the expression of Cathepsin K in RANKL stimulated RAW 264.7 cells. 32
Fig. 8. Effects of Brazilin on activation of MAP kinases in RANKL-stimulated RAW264.7 cells. 34
Fig. 9. ¹H-NMR spectra of brazilin (DMSO-d6, δ 0 ~ 12ppm)(이미지참조) 36
Fig. 10. ¹H-NMR spectra of brazilin (DMSO-d6, δ 6.1 ~ 7.2ppm)(이미지참조) 37
Fig. 11. ¹H-NMR spectra of brazilin (DMSO-d6, δ2.4 ~ 4.0ppm)(이미지참조) 38
Fig. 12. ¹³C-NMR spectra of brazilin (DMSO-d6, δ 0 ~ 220ppm)(이미지참조) 39
Fig. 13. Low-resolution FAB-MS spectra of brazilin 41
Fig. 14. Molecular weight of brazilin measured by high-resolution FAB-MS 42
Fig. 15. Chemical structure of brazilin(7,11b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10 (6H)-tetrol) 43