Title Page
국문초록
Contents
I. Introduction 9
II. Materials and method 11
1. Materials 11
2. Cell culture 11
3. Cell viability assay 12
4. Reverse transcription (RT)-PCR 13
5. Western blot analysis 14
6. Apoptosis evaluation 14
7. Data analysis 15
III. Results 16
1. Effect of bee venom on cell growth in ovarian cancer cells 16
2. Apoptotic cell death by bee venom 19
3. Expression of death receptors in ovarian cancer cells by bee venom 22
4. Effect of bee venom on the expression of apoptotic regulatory proteins 24
5. Reversed effect of DR siRNAs on bee venom-induced cell growth inhibition 26
6. Association between death receptor and activation of STAT3 30
IV. Discussion 33
References 36
ABSTRACT 42
Fig. 1. Effect of bee venom on cell viability in ovarian cancer cells. 17
Fig. 2. Effect of bee venom on cell viability in ovarian cancer cells. 18
Fig. 3. Effect of bee venom on apoptotic cell death. 20
Fig. 4. Effect of bee venom on apoptotic cell death. 21
Fig. 5. Effect of bee venom on DRs expression in ovarian cancer cells. 23
Fig. 6. Effect of bee venom on the expression of apoptosis regulatory proteins. 25
Fig. 7. Effect of siRNA of DRs on the bee venom-induced cancer cell growth inhibition, DR expression and apoptosis in ovarian cancer cells. 27
Fig. 8. Effect of DR3, DR4, or DR6 knockdown on bee venom-induced ovarian cancer cell growth. 28
Fig. 9. Quantification of apoptosis by TUNEL assay. 29
Fig. 10. Effect of bee venom and siRNA of DRs on the activation of JAK2/STAT3 in ovarian cancer cells. 31
Fig. 11. Ovarian cancer cells were transfected with nontargeting control siRNA, DR3, DR4, or DR6 siRNA(100nM) as described in Materials and Methods for 24hr. 32