Title Page
국문초록
Contents
I. Introduction 9
II. Materials and Methods 11
1. Materials 11
2. Cell Culture 11
3. Cell Viability Assay 11
4. Cell Cycle Analysis 12
5. Reverse transcription(RT)-PCR 13
6. Western blot analysis 13
7. Apoptosis evaluation 14
8. Data analysis 15
III. Results 16
1. Effect of SVT Toxin on Cell Growth in HCT116 Colon Cancer Cells 16
2. Expression of Death Receptors in HCT116 Colon Cancer Cells by SVT 18
3. Cell Cycle Arrest at the G0/G1 and G2/M Phase(이미지참조) 20
4. Apoptotic Cell Death by SVT 22
5. Effect of SVT on the Expression of Apoptotic Regulatory Proteins 24
6. Effect of SVT on the Activation of NF-κB Signal Molecules and STAT3 26
IV. Discussion 28
References 32
ABSTRACT 39
Fig. 1. Effect of SVT on Cell Viability in HCT116 Colon Cancer Cells 17
Fig. 2. Effect of SVT on DRs Expression in HCT116 Colon Cancer Cells 19
Fig. 3. Cell Cycle Analysis of HCT116 Colon Cancer Cells Treated with Various Doses of SVT. 21
Fig. 4. Effect of SVT on Apoptotic Cell Death of HCT116 Colon Cancer Cells. 23
Fig. 5. Effect of SVT on the Expression of Apoptosis Regulatory Proteins. 25
Fig. 6. Effect of SVT on the Activation of NF-κB/STAT3 in HCT116 Colon Cancer Cells 27