표제지
목차
I. 서론 7
II. 재료 및 실험방법 11
1. 시약 11
2. 정유 추출 방법 11
3. 행인 정유의 성분분석 13
4. Tyrosinase 활성 억제효과 13
5. 사용균주 14
6. 디스크 확산법 16
7. 최소억제농도(MIC, Minimum Inhibitory Concentration) 측정 17
III. 결과 및 고찰 19
1. 행인 정유의 추출율 19
2. 행인 정유의 성분 19
3. Mushroom tyrosinase 억제효과 측정 19
4. 항균활성 측정 20
1) 디스크 확산법 20
2) 최소억제농도(MIC) 21
IV. 결론 23
V. 참고문헌 35
초록 39
Abstract 41
〈Table 1〉 Measurement condition of GC-MS 13
〈Table 2〉 Gram-positive bacteria strains 14
〈Table 3〉 Gram-negative bacteria strains 15
〈Table 4〉 Yeast strain 15
〈Table 5〉 Modified Leeming and Notman medium (1liter) 17
〈Table 6〉 Chemical composition of Armeniacae semen essential oil 25
〈Table 7〉 Effects of Armeniacae semen essential oil and other materials against mushroom tyrosinase 30
〈Table 8〉 Antimicrobial actibities of Armeniacae semen essential oil and other materials against Gram-positive bacteria using the disk diffusion tests. 30
〈Table 9〉 Antimicrobial actibities of Armeniacae semen essential oil and other materials against Gram-negative bacteria using the disk diffusion tests. 31
〈Table 10〉 Antimicrobial actibities of Armeniacae semen essential oil and other materials against yeast using the disk diffusion tests. 32
〈Table 11〉 MICs of Armeniacae semen essential oil for Gram-positive bacteria 32
〈Table 12〉 MICs of Armeniacae semen essential oil for Gram-negative bacteria 33
〈Table 13〉 MICs of Armeniacae semen essential oil for yeast 34
〈Fig. 1〉 Clevenger type apparatus. 12
〈Fig. 2〉 GC-MS chromatogram of Armeniacae semen essential oil. 24
〈Fig. 3〉 Time course of DOPAchrome formation in the presence of 0.6μg/ml of Armeniacae semen essential oil. 26
〈Fig. 4〉 Effect of Armeniacae semen essential oil against mushroom tyrosinase. 26
〈Fig. 5〉 Effect of kojic acid against mushroom tyrosinase. 27
〈Fig. 6〉 Effect of ascorbic acid against mushroom tyrosinase. 27
〈Fig. 7〉 Effect of Citrus limon L. essential oil against mushroom tyrosinase. 28
〈Fig. 8〉 Effect of Thuja orientalis essential oil against mushroom tyrosinase. 28
〈Fig. 9〉 Effect of benzaldehyde against mushroom tyrosinase. 29
〈Fig. 10〉 Effect of benzenemethanol against mushroom tyrosinase. 29