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목차
I. 서론 12
II. 재료 및 방법 15
1. 실험 재료 15
1) 재료 15
2) 시료 추출 15
3) 시약 및 기기 17
2. 실험 방법 19
1) 항산화 효과 측정 19
2) 총 폴리페놀 함량 측정 23
3) 미백 효과 측정 24
4) 주름개선 효과 측정 25
5) 수렴 효과 측정 27
6) 항균 효과 측정 28
7) W/O/W multiple emulsion 제조 30
8) 감꽃 추출물을 첨가한 W/O/W multiple emulsion의 안정성 측정 33
9) 감꽃 추출물을 첨가한 W/O/W multiple emulsion의 유효성 측정 37
10) 감꽃 추출물을 첨가한 W/O/W multiple emulsion의 안전성 측정 38
III. 결과 및 고찰 39
1. 항산화능 측정결과 39
1) 전자공여 능 확인 39
2) Superoxide dismutase(SOD) 유사활성 확인 41
3) ABTS+ Radical cation decolorization assy(이미지참조) 43
4) Xanthine oxidase 저해활성 확인 45
2. 총 Polyphenol 함량 확인 47
3. 미백 효과 측정 결과 49
1) Tyrosinase 저해 활성 확인 49
4. 주름개선 효과 측정 결과 51
1) Elastase 저해활성 확인 51
2) Collagenase 저해활성 확인 53
5. 수렴 효과 측정 결과 55
1) Astringent 활성 측정 55
6. 항균 효과 측정 결과 57
1) 생육 저해 환(clear zone) 확인 57
7. 감꽃 추출물을 첨가한 화장품의 안정성 측정 결과 62
1) 감꽃 추출물을 첨가한 W/O/W multiple emulsion 입자 안정성 확인 62
8. 감꽃 추출물을 첨가한 W/O/W multiple emulsion의 유효성 측정결과 72
1) 감꽃 추출물을 첨가한 W/O/W multiple emulsion의 항산화능 측정 결과 72
2) 감꽃 추출물을 첨가한 W/O/W multiple emulsion의 주름 효과 측정 결과 74
9. 감꽃추출물을 첨가한 화장품의 안전성 측정결과 76
1) 첩포시험 76
IV. 참고문헌 78
초록 86
Abstract 88
Table 1. The experimental formulation of the W/O/W multiple emulsion containing Persimmon flowers extracts. 31
Table 2. Check points in stability test of the W/O/W multiple emulsion containing Persimmon flowers extracts in constant temperature condition (0, 25, 40℃). 35
Table 3. The expression method of patch test 38
Table 4. The contents of total polyphenol of persimmon flowers extract. 48
Table 5. Antimicrobial activity of Persimmon flowers extracts on several microorganisms. 58
Table 6. pH measurement of Persimmon flowers extracts. 64
Table 7. Result of stability test of the W/O/W multiple emulsion containing Persimmon flowers extracts in constant temperature conditions(0, 25, 40℃). 69
Table 8. Result of stability test of the W/O/W multiple emulsion containing Persimmon flowers extracts in cycle chamber condition. 71
Table 9. Result of patch test of the W/O/W multiple emulsion containing Persimmon flowers extracts. 76
Fig. 1. The Procedure for extraction from persimmon flowers. 16
Fig. 2. Preparation of the W/O/W multiple emulsion containing for Persimmon flowers extracts. 32
Fig. 3. Cycle temperature change with day for 1 cycle.(-15℃, -10℃, 0℃, -5℃, 0℃, 5℃, 10℃, 15℃, 25℃, 37.5℃, 40℃) 36
Fig. 4. Electron donating ability of Persimmon flowers extracts. 40
Fig. 5. SOD-like activity of Persimmon flowers extracts. 42
Fig. 6. ABTS+ radical cation scavenging activity of Persimmon flowers extracts.(이미지참조) 44
Fig. 7. Inhibition rate of Persimmon flowers extracts on xanthine oxidase. 46
Fig. 8. Inhibition rate of Persimmon flowers extracts on tyrosinase. 50
Fig. 9. Inhibition rate of Persimmon flowers extracts on elastase. 52
Fig. 10. Inhibition rate of Persimmon flowers extracts on collagenase. 54
Fig. 11. Comparison of astrigent activity of Persimmon flowers extracts. 56
Fig. 12. Antimicrobial activity of Persimmon flowers water extracts on several microorganisms. 59
Fig. 13. Antimicrobial activity of Persimmon flowers ethanol extracts on several microorganisms. 60
Fig. 14. Antimicrobial activity of Persimmon flowers acetone extracts on several microorganisms. 61
Fig. 15. Microscopic aspect of the W/O/W multiple emulsion containing Persimmon flowers extracts (X1000). 63
Fig. 16. pH measurement of the W/O/W multiple emulsion containing Persimmon flowers acetone extracts. 65
Fig. 17. Viscosity change of the W/O/W multiple emulsion containing Persimmon flowers acetone extracts. 67
Fig. 18. Electron donating ability of the MLV liposomes containing n-BuOH layer of Persimmon flowers extracted with acetone. 73
Fig. 19. Elastase inhibition activity of the MLV liposomes containing n-BuOH layer of Persimmon flowers extracted with acetone. 75
Fig. 20. Photograph of patch test of the W/O/W multiple emulsion containing Persimmon flowers extracts. 77