Title Page
Contents
국문초록 9
I. Introduction 12
II. Materials and Methods 17
1. Samples, chemicals, and instruments 17
1.1. Preparation of fermented beverage of plant extracts 17
1.2. Chemicals and instruments 17
1.3. Bacteria, plasmids, and media 18
2. Chemical analysis 19
2.1. pH and acidity 19
2.2. Brix and reducing sugar 19
2.3. Alcohol 20
2.4. Free sugar 20
2.5. Free organic acid 21
2.6. Soluble phenolics and flavonoids 22
3. Recombinant DNA techniques 24
4. Enrichment culture and DNA extraction 24
5. PCR amplification 26
5.1. 16S ribosomal DNA 26
5.2. 26S ribosomal DNA 27
6. Ribosomal DNA library construction 28
7. DNA sequencing and sequence analysis 28
III. Results 30
1. Physicochemical prosperities of FBPEs 30
2. Detection of 16S rDNA and 26S rDNA 32
3. Bacterial diversity 35
3.1. Bacterial diversity from an enrichment culture of FBPEs 35
3.2. Phylogenetic placement of bacteria on based on 16S rDNA sequences 43
3.3. Bacterial distribution of the three libraries 45
4. Yeast diversity 46
4.1. Yeast diversity from an enrichment culture of FBPEs 46
4.2. Phylogenetic placement of bacteria on based on 26S rDNA sequences 56
4.3. Yeast distribution of the three libraries 58
IV. Discussion 60
V. References 68
ABSTRACT 80
Appendix 83
Appendix I. 16S rDNA sequences 83
Appendix II. 26S rDNA sequences 85
Table 1. Characterization of physicochemical prosperities in... 31
Table 2. Similarity values of 16S rRNA sequences retrieved... 37
Table 3. Similarity values of 16S rRNA sequences retrieved... 39
Table 4. Similarity values of 16S rRNA sequences retrieved... 41
Table 5. Lactic acid bacterial distribution of the three libraries... 45
Table 6. Similarity values of 26S rRNA sequences retrieved... 50
Table 7. Similarity values of 26S rRNA sequences retrieved... 52
Table 8. Similarity values of 26S rRNA sequences retrieved... 54
Table 9. Yeast distribution of the three libraries from... 59
Table 10. Overview of relationship between phylum and... 66
Fig. 1. Flow diagram of the integrated approach used in... 25
Fig. 2. Electrophoresis of PCR products when the primers... 33
Fig. 3. Electrophoresis of PCR products when the primers... 34
Fig. 4. Phylogenetic placement of 16S rDNA sequences from... 44
Fig. 5. Phylogenetic placement of 26S rDNA sequences from... 57