표제지
抄錄
목차
I. 서론 11
II. 재료 및 방법 13
1. 재료 13
1) 약재 13
2) 동물 13
2. 방법 14
1) 실험군 분류 및 아토피 유발 14
2) 약물처리 14
3) 혈액 및 장기 적출 15
4) 혈액 분석 15
5) 조직 절편 제조 15
6) 피부 조직 염색 16
7) 혈중 IgE 농도 분석 17
8) 비장 조직 cDNA 합성 17
9) 비장의 cytokine의 발현 분석 18
10) 통계처리 20
III. 결과 21
1. 실험동물의 체중에 미치는 영향 21
2. 혈액 분석 결과 22
1) 적혈구에 미치는 영향 23
2) 혈소판에 미치는 영향 24
3) 백혈구에 미치는 영향 25
4) 림프구에 미치는 영향 26
5) 단핵백혈구에 미치는 영향 27
6) 호중구에 미치는 영향 28
7) 호산구에 미치는 영향 29
3. 피부 조직에 미치는 영향 30
1) 조직학적 특성에 미치는 영향 30
2) T세포에 미치는 영향 34
4. 혈액 내 IgE 분석 결과 35
5. 비장의 사이토카인 발현에 미치는 영향 36
1) IL-4 발현에 미치는 영향 36
2) IL-6 발현에 미치는 영향 37
3) TNF-α 발현에 미치는 영향 38
4) IFN-γ 발현에 미치는 영향 39
IV. 고찰 41
V. 결론 47
참고문헌 48
ABSTRACT 52
Table 1. The sequence of PCR primers. 19
Fig. 1. A variation of acute atopic dermatitis induced mice body weight. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. Each value represents the mean±SD. 21
Fig. 2. Effects of herbal mixture on levels of blood RBCs in acute atopic dermatitis induced mice. Each bar represents the mean±SD of RBCs levels in blood. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration... 23
Fig. 3. Effects of herbal mixture on levels of blood platelets in acute atopic dermatitis induced mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. Each bar represents the mean±SD of platel-... 24
Fig. 4. Effects of herbal mixture on levels of blood white blood cells in acute atopic dermatitis induced mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. Each bar represents the... 25
Fig. 5. Effects of herbal mixture on levels of blood lymphocytes in acute atopic dermatitis induced mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. Each bar represents the mean±SD of... 26
Fig. 6. Effects of herbal mixture on levels of blood monocytes in acute atopic dermatitis induced mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. Each bar represents the mean±SD of... 27
Fig. 7. Effects of herbal mixture on levels of blood neutrophils in acute atopic dermatitis induced mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. Each bar represents the mean±SD of... 28
Fig. 8. Effects of herbal mixture on levels of blood eosinophils in acute atopic dermatitis induced mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. Each bar represents the mean±SD of... 29
Fig. 9. Comparison of the histological feature of hematoxylin and eosin-stained dorsal skin sections in acute atopic dermatitis mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. 31
Fig. 10. Comparison of the hematoxylin-stained cells in acute atopic dermatitis mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. 32
Fig. 11. Comparison of hematoxylin-stained cell count in the lesional skin of acute atopic dermatitis mice(** : p〈0.01 vs negative control group). Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. 33
Fig. 12. Comparison of the histological feature of CD3 immunostained dorsal skin sections in acute atopic dermatitis mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. 34
Fig. 13. Effects of herbal mixture on levels of blood IgE in acute atopy dermatitis induced mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. 35
Fig. 14. The levels of IL-4 mRNA in spleen from mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. 36
Fig. 15. The levels of IL-6 mRNA in spleen from mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. 37
Fig. 16. The levels of TNF-α mRNA in spleen from mice(** : p〈0.01 vs negative control group). Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. 38
Fig. 17. The levels of IFN-γ mRNA in spleen from mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. 39
Fig. 18. The levels of IFN-γ/TNF-α mRNA in spleen from mice. Non-treated normal group is Normal, non-treated control group is Control, herbal mixture apply to the back skin group is DAHM, and oral administration group is OAHM. 40