표제지
抄錄
목차
I. 서론 12
II. 재료 및 방법 15
1. 재료 15
1) 약재 추출 15
2) 연고 조제 16
3) 실험동물 16
2. 방법 18
1) 연구 디자인 및 화상유발 18
2) 화상유발 동물에서의 가미자운고의 처치 18
3) 조직학적 검사 (Histochemical study) 19
4) 화상부위 크기 측정 19
5) 전혈 채취 및 혈액학적 분석 19
6) Western blot analysis 20
3. 통계처리 21
III. 결과 22
1. 2도 화상 모델의 조직학적 확인 22
2. 화상 개선효과 24
3. 화상의 조직학적 개선효과 27
4. 백혈구 수치에 미치는 영향 29
5. 적혈구 수치에 미치는 영향 30
6. 헤모글로빈 수치에 미치는 영향 31
7. 혈소판 수치에 미치는 영향 32
8. 헤마토크리트 수치에 미치는 영향 33
9. 혈관신생관련 단백질 발현에 미치는 영향 34
IV. 고찰 36
결론 42
참고문헌 44
ABSTRACT 49
Table 1. Prescription of Gamijaungo 16
Table 2. Classification by treatments 18
Figure 1. Histological evaluation of preconditioning. Hematoxylin and eosin (H&E) stains 12 hours after treatment (scale bar=100 um) (A) Normal tissue shows a healthy epidermis and hair follicles. (B) Untreated control of burn-induced tissues shows ablation of the... 23
Figure 2. Morphologic feature of skin of burned mouse back ofuntreated control group, C (A) and treated with Gamijaungo treated group, T (B) 25
Figure 3. Size of impaired skin of burned mouse back of untreated control group, C (A) and treated with Gamijaungo group, T (B) on the 15th day of experiment. 26
Figure 4. Histological skin features ofuntreated and treated sites on theburn-induced lesionsin mice. Photomicrographs of the sites from untreated (A) and Gamijaungo-treated skin on the 15th day after treatment (B) in mice. Lesional skin was harvested and examined by... 28
Figure 5. Hematologic changes of White Blood Cell in the burn-induced mice. Values are expressed as mean±standard deviation (mean±S.D.), and analyzed by one-way ANOVA and multiple comparisons were performed by Tukey HSD test. A criterion of... 29
Figure 6. Hematologic changes of Red Blood Cell in the burn-induced mice. Values are expressed as mean±standard deviation (mean±S.D.), and analyzed by one-way ANOVA and multiple comparisons were performed by Tukey HSD test. A criterion of... 30
Figure 7. Hematologic changes of Hemoglobin in the burn-induced mice. Values are expressed as mean±standard deviation (mean±S.D.), and analyzed by one-way ANOVA and multiple comparisons were performed by Tukey HSD test. A criterion of significance was accepted and marked... 31
Figure 8. Hematologic changes of Platelet in the burn-induced mice. Values are expressed as mean±standard deviation (mean±S.D.), and analyzed by one-way ANOVA and multiple comparisons were performed by Tukey HSD test. A criterion of significance was... 32
Figure 9. Hematologic changes of hematocrit in the burn-induced mice. Values are expressed as mean±standard deviation (mean±S.D.), and analyzed by one-way ANOVA and multiple comparisons were performed by Tukey HSD test. A criterion of significance was... 33
Figure 10. Effects of Gamijaungo on VEGF, PI3K and pAkt protein expression (A) and the inhibitory percentage of VEGF, PI3K and pAkt expression (B). Values are expressed as mean±standard deviation (mean±S.D.), and analyzed by one-way ANOVA and multiple... 34