Title Page
ABSTRACT
Contents
I. Introduction 13
II. Materials and Methods 16
1. Reagents 16
2. Instruments 16
3. Preparation of Lycii Fructus(= LF) extract. 17
4. Cell culture 17
5. Cell viability 18
6. Quantification of nitric oxide production 18
7. Multiplex bead-based cytokine assay 19
8. Intracellular calcium assay 20
9. Statistical analysis 21
III. Results 22
1. Cell viability 22
1) Effect of LF on cell viability 22
2) Effect of LF on LPS-induced cell viability 25
2. Effect on NO production 27
3. Effect on cytokine production 29
1) IL-10 29
2) IL-17 32
3) IL-12p40 34
4) IL-12p70 36
5) IP-10 38
6) KC 40
7) MCP-1 42
8) M-CSF 44
9) VEGF 46
10) GM-CSF 48
11) IL-4 50
12) MIP-2 52
4. Effects of LF on intracellular calcium production 54
IV. Discussion 56
V. Conclusions 63
References 65
국문초록 81
Table 1. Effect of LF on Cell Viability in RAW 264.7 Cells for 24 hr Incubation 23
Table 2. Effect of LF on LPS-induced Cell Viability in RAW 264.7 Cells for 24 hr Incubation. 25
Table 3. Effect of LF on NO Production in LPS-activated RAW 264.7 Cells. 27
Table 4. Effect of LF on IL-10 Production in LPS-activated RAW 264.7 Cells. 30
Table 5. Effect of LF on IL-17 Production in LPS-activated RAW 264.7 Cells. 32
Table 6. Effect of LF on IL-12p40 Production in LPS-activated RAW 264.7 Cells. 34
Table 7. Effect of LF on IL-12p70 Production in LPS-activated RAW 264.7 Cells. 36
Table 8. Effect of LF on IP-10 Production in LPS-activated RAW 264.7 Cells. 38
Table 9. Effect of LF on KC Production in LPS-activated RAW 264.7 Cells. 40
Table 10. Effect of LF on MCP-1 Production in LPS-activated RAW 264.7 Cells. 42
Table 11. Effect of LF on M-CSF Production in LPS-activated RAW 264.7 Cells. 44
Table 12. Effect of LF on VEGF Production in LPS-activated RAW 264.7 Cells. 46
Table 13. Effect of LF on GM-CSF Production in LPS-activated RAW 264.7 Cells. 48
Table 14. Effect of LF on IL-4 Production in LPS-activated RAW 264.7 Cells. 50
Table 15. Effect of LF on MIP-2 Production in LPS-activated RAW 264.7 Cells. 52
Table 16. Effects of LF on Intracellular Calcium in LPS-activated RAW 264.7 cells. 54
Fig. 1. Effect of LF on cell viability in RAW 264.7 cells for 24 hr incubation. 24
Fig. 2. Effect of LF on LPS-induced Cell Viability in RAW 264.7 Cells for 24 hr Incubation. 26
Fig. 3. Effect of LF on NO Production in LPS-activated RAW 264.7 Cells. 28
Fig. 4. Effect of LF on IL-10 Production in LPS-activated RAW 264.7 Cells. 31
Fig. 5. Effect of LF on IL-17 Production in LPS-activated RAW 264.7 Cells. 33
Fig. 6. Effect of LF on IL-12p40 Production in LPS-activated RAW 264.7 Cells. 35
Fig. 7. Effect of LF on IL-12p70 Production in LPS-activated RAW 264.7 Cells. 37
Fig. 8. Effect of LF on IP-10 Production in LPS-activated RAW 264.7 Cells. 39
Fig. 9. Effect of LF on KC Production in LPS-activated RAW 264.7 Cells. 41
Fig. 10. Effect of LF on MCP-1 Production in LPS-activated RAW 264.7 Cells. 43
Fig. 11. Effect of LF on M-CSF Production in LPS-activated RAW 264.7 Cells. 45
Fig. 12. Effect of LF on VEGF Production in LPS-activated RAW 264.7 Cells. 47
Fig. 13. Effect of LF on GM-CSF Production in LPS-activated RAW 264.7 Cells. 49
Fig. 14. Effect of LF on IL-4 Production in LPS-activated RAW 264.7 Cells. 51
Fig. 15. Effect of LF on MIP-2 Production in LPS-activated RAW 264.7 Cells. 53
Fig. 16. Effects of LF on intracellular calcium in LPS-activated RAW 264.7 cells. 55