표제지
목차
Abbreviations 10
I. 서론 11
II. 재료 및 방법 13
1. 실험재료 13
1) 본 실험에 사용된 녹차는 경남 산청군 시천면 내대리 보명 다원 농장에서 자란 자연산 재래종이며, 불발효차로서 찻잎을 따는 시기에 따라 Table 1과 같이 곡우를 전후하여 따기 시작하는 우전(雨前)에서 세작(細作), 중작(中作)까지 농장에서 2010... 13
2) 녹차의 가공 14
2. 실험설계 및 조사방법 16
1) 실험설계 16
2) 엽록소 추출 및 정량 16
3) TLC(Thin Layer Chromatography) 조건 18
4) 색소분석 20
5) Carotenoids의 분리 및 정량 20
3. 생물학적 실험 22
1) 녹차 추출액의 항균시험 22
2) 동물성 플랑크톤을 이용한 녹차 추출액의 독성실험 24
4. 통계 분석 24
III. 결과 및 고찰 25
1. TLC를 통한 carotenoids의 분리 25
2. 채엽시기에 따른 엽록소함량의 변화 34
3. 채엽시기에 따른 carotenoids 함량의 변화 36
1) β-carotene 함량의 변화 36
2) Lutein 함량의 변화 42
4. 녹차 추출액의 항균시험 47
5. 동물성 플랑크톤을 이용한 녹차 추출액의 독성실험 49
IV. 결론 51
참고문헌 52
Abstract 57
Table 1. Common classification of green tea named by different harvesting time 13
Fig. 1. The scheme of procedure for green tea processing. 15
Fig. 2. The sheme of isolation of chlorophyll and fixed quantity of chlorophyll for TLC analysis of carotenoids. 18
Fig. 3. Schematic representation of comprehensive two-dimensional chromatography on one adsorbent. A, spotting a sample at the corner of a chromatographic plate; B, development of the plate in... 19
Fig. 4. The calibration curve of standard β-carotene. The reagent purchased from FLUCA Co. for UV, purum ≥97.0% 21
Fig. 5. The calibration curve of standard lutein. The reagent purchased from SIGMA Co. for UV, purum ≥95.0% 22
Fig. 6. The scheme of procedure on control test of putrefactive bacteria by green tea extracts. 23
Fig. 7. TLC photograph of carotenoid developed with the ratio 40 to 20 of n-hexane to acetone in development solvent. A. Fresh Leaves; B. Standards[β-carotene(up) and lutein]; C. Woo-Jeon; D. Se-Jak; E. Jung-Jak, and... 28
Fig. 8. TLC photograph of carotenoid developed with the ratio 50 to 50 of n-hexane to acetone in development solvent. A. Fresh Leaves; B. Standards[β-carotene(up) and lutein]; C. Woo-Jeon; D. Se-Jak; E. Jung-Jak, and... 29
Fig. 9. TLC photograph of carotenoid developed with the ratio 70 to 30 of n-hexane to acetone in development solvent. A. Fresh Leaves; B. Standards[β-carotene(up) and lutein]; C. Woo-Jeon; D. Se-Jak; E. Jung-Jak, and... 30
Fig. 10. TLC photograph of carotenoid developed with the ratio 85 to 15 of n-hexane to acetone in development solvent. A. Fresh Leaves; B. Standards[β-carotene(up) and lutein]; C. Woo-Jeon; D. Se-Jak; E. Jung-Jak, and... 31
Fig. 11. TLC photograph of carotenoid developed with the ratio 85 to 15 of n-hexane to acetone in development solvent for 90 mins and then done with another ratio 50 to 50 for 80 mins. A. Fresh Leaves; B. Standards... 32
Fig. 12. TLC photographs of 2D of carotenoid developed with the ratio 50 to 50 of n-hexane to acetone for 50 mins after done with ratio 40 to 20 in development solvent for 90 mins. A. Fresh Leaves; B. Woo-Jeon; C. Se-Jak;... 33
Fig. 13. Chlorophyll contents of green tea by different harvesting periods. 35
Fig. 14. Absorption spectra of β-carotene in acetone. 1. Fresh Leaves; 2. Jung-Jak; 3. Standard; 4. Woo-Jeon and 5. Se-Jak. 38
Fig. 15. β-carotene content in green tea leaves by different harvesting periods. 40
Fig. 16. β-carotene content per total chlorophyll in green tea leaves by different harvesting periods. 41
Fig. 17. Absorption spectra of lutein in EtOH. 1. Standard; 2. Jung-Jak; 3. Fresh Leaves; 4. Se-Jak and 5. Woo-Jeon. 44
Fig. 18. Lutein content in green tea leaves by different harvesting periods. 45
Fig. 19. Lutein content per total chlorophyll in green tea leaves by different harvesting periods. 46
Fig. 20. Antibacterial effect of different concentration of extract from green teas on Maggolli putrefactive bacteria after incubation for 48-h by different harvesting periods. 48
Fig. 21. The survival number of Artemia after incubation for 24-h in different concentration of extract from green teas by different harvesting periods. 50