표제지
목차
Abbreviations 8
I. 서론 9
II. 재료 및 방법 15
1. 실험재료 15
1) 식물재료 15
2) 시약 15
3) 검액의 조제 15
4) 실험동물 16
2. RAPD PCR 분석 17
1) Genomic DNA 분리 17
2) RAPD PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) 분석 18
3) DNA 전기영동(Electrophoresis) 18
3. 항산화력 측정 19
4. Acetylcholinesterase 저해력 측정 19
5. 수동회피시험(passive avoidance test) 20
6. Y-미로시험(Y-maze test) 21
7. TPA 유도 급성피부염 억제효과 22
8. 초산신전반응 (Writing test) 22
9. 통계처리 23
III. 결과 및 고찰 24
1. RAPD 유연관계 분석 24
2. 항산화실험 26
3. AchE 저해능 실험 26
4. 수동회피실험(Passive Avoidance Test) 28
5. Y-미로시험 31
6. 급성접촉성피부염시험(TPA) 33
7. 초산신전반응 35
IV. 결론 37
참고문헌 39
Abstract 46
Table 1. Morphological studies on native and naturalized Taraxacum species 12
Table 2. Test sample named by different Species and harvesting time. 16
Table 3. Number of PCR products in Taraxacum coreanum and T.officinale. 25
Table 4. IC50 value of Free radical scavenging activity and Acetylcholine Esterease inhibitory effects on Taraxacum coreanum and T. officinale. 27
Fig. 1. DNA amplication patterns obtained by Random primer 1 and 2. 25
Fig. 2. Inhibitory effects of TE on scopolamine-induced memory impairment in mice in the Passive avoidance test. 30
Fig. 3. Inhibitory effects of TE on scopolamine-induced memory impairment in mice in the Y-maze test. 31
Fig. 4. The effects of TE on the ear thickness of mice induced by TPA 34
Fig. 5. Inhibitory effects of TE on acetic acid induced writhing syndromes in mice 36