표제지
목차
Abbreviations 7
I. 서론 8
II. 연구사 13
III. 재료 및 방법 17
1. 사양실험 및 육질 분석 17
1) 실험동물과 실험사료 분석 17
2) 육질분석 18
2. 유전자 발현 분석 20
1) Total RNA 분리 20
2) RNA quality test 및 cDNA 합성 22
3) Transcriptome 분석 24
IV. 결과 및 고찰 30
1. 발효사료 급여에 따른 육질형질 분석 결과 30
2. 차등 유전자 발현 분석 32
V. 참고문헌 41
Abstract 45
Table 1. Data for meat quality traits 19
Table 2. Profiling of differentially expressed genes in pig fed fermented feed. 33
Table 3. Representative up-regulated genes 36
Table 4. Representative down-regulated genes 39
Figure 1. Quality and quantify analysis of total RNA isolated from pigs fed fermented feed. (a) total RNA isolated from pigs fed general feed, (b) total RNA isolated from pigs fed fermented feed 21
Figure 2. RNA quality test by Bioanalyzer 2100 gel image and electropherogram analyses. (a) RNA quality test by Bioanalyzer 2100 gel image analysis, (b) RNA quality test by Bioanalyzer 2100 electropherogram analysis 23
Figure 3. Microarray hybridization image scan result. (a) Hybridization of RNAs isolated from pigs fed general feed, (b) Hybridization of RNAs isolated from pigs fed fermented feed. 25
Figure 4. Pipeline of microarray analysis 26
Figure 5. PCR confirmation of microarray hybridization 28
Figure 6. Ontology classification of total 1,240 gene 33
Figure 7. Ontology classification of 878 up-regulated genes 35
Figure 8. Ontology classification of 362 down-regulated genes 38