표제지
목차
I. 서론 6
II. 재료 및 방법 8
1. 실험 재료 8
2. 추출물 제조 8
(1) 지각 환류 물 추출물(PTW; P. trifoliata Reflux water extract) 8
(2) 지각 환류 에탄올 추출물(PTE; P. trifoliata Reflux ethanol extract) 8
3. 시약 및 기기 11
4. 폴리페놀 화합물 함량(polyphenol compound contents) 12
5. 플라보노이드 화합물 함량(flavonoid compound contents) 13
6. SOD 유사활성능 (Superoxide dismutase like activity) 14
7. 전자공여능 (Electron donating ability: EDA) 15
8. 아질산염 소거능 (Nitrite-scavenging ability: NSA) 16
9. 통계처리 17
III. 결과 및 고찰 18
1. 고형분 수율 18
2. 폴리페놀 화합물 함량 21
3. 플라보노이드 화합물 함량 24
4. SOD 유사활성능 27
5. 전자공여능 30
6. 아질산염 소거능 33
IV. 결론 38
참고문헌 40
(국문초록) 46
〈Abstract〉 47
Table 1. Extraction yield of water and ethanol extracts of P. trifoliata 19
Table 2. Total polyphenol compound contents of water and ethanol extracts of P. trifoliata 22
Table 3. Total flavonoid compound contents of water and ethanol extracts of P. trifoliata 25
Table 4. Superoxide dismutase (SOD)-like activities of water and ethanol extracts of P. trifoliata 28
Table 5. Electron donating abilities of water and ethanol extracts of P. trifoliata 31
Table 6. Nitrite scavenging abilities of water and ethanol extracts of P. trifoliata at various pH condition. 35
Fig. 1. Extraction procedure of the fruit of P. trifoliata. 9
Fig. 2. Procedure experiment of P. trifoliata. 10
Fig. 3. The method of polyphenol contents measurement. 12
Fig. 4. The method of flavonoid contents measurement. 13
Fig. 5. The method of superoxide dismutase (SOD) like ability measurement. 14
Fig. 6. The method of electron donating ability measurement. 15
Fig. 7. The method of nitrite scavenging ability measurement. 16
Fig. 8. Extraction yield of water and ethanol extracts of P. trifoliata. 20
Fig. 9. Total polyphenol compound contents of water and ethanol extracts of P. trifoliata. 23
Fig. 10. Total flavonoid compound contents of water and ethanol extracts from P. trifoliata. 26
Fig. 11. SOD-like activities of water and ethanol extracts of P. trifoliata 29
Fig. 12. Electron donating abilities of water and ethanol extracts of P. trifoliata 32
Fig. 13. Nitrite scavenging abilities of water and ethanol extracts of P.trifoliata at pH 1.2(a), 3.0(b) and 6.0(c) condition. 37