표제지
목차
I. 緖論 9
II. 材料 및 方法 15
1. 材料 15
(1) 實驗動物 15
(2) 試料의 준비 15
(3) 試驗裝置 16
(4) 試驗物質 17
2. 方法 18
(1) 檢液의 調製 18
(2) 행동약리학적 연구 18
3. 統計處理 23
III. 實驗結果 24
1. Elevated Plus-maze에서 지황 추출물의 항불안 효과 24
(1) 각 arm에서 머문 시간 24
(2) 각 arm으로의 출입횟수 26
(3) Open arm에서 머문 시간 및 출입한 횟수의 백분율 28
(4) 총 이동거리의 측정 32
2. WAY 100635 및 flumazenil의 길항작용 34
3. Horizontal wire test 37
4. Locomotor activity test 39
5. Forced swimming test (FST)를 통한 지황 추출물의 항우울 작용 확인 42
6. Tail suspension test를 통한 지황 추출물의 항우울작용 확인 45
IV. 考察 48
V. 結論 53
參考文獻 55
국문초록 60
ABSTRACT 62
Table 1. Time spent in the behaviors shown by mice exploring the elevated plus-maze (mean ± S.E.M.) 25
Table 2. Frequency in the behaviors shown by mice exploring the elevated plus-maze (mean ± S.E.M.) 27
Table 3. General activity measures from plus maze test (mean ± S.E.M.) 29
Table 4. Total distance moved in closed arms and open arms of elevated plus-maze over 5-min test in mice. (mean ± S.E.M.) 33
Table 5. Antagonism study from plus maze test (mean±S.E.M.) 35
Table 6. The effects of Rehmannia glutinosa on the locomotor activity in the open field test 40
Table 7. Inhibitory effect of Rehmannia glutinosa on the duration of immobility by forced swimming test. 43
Table 8. Inhibitory effect of Rehmannia glutinosa on the duration of immobility by tail suspension test. 46
Figure 1. Three types of Rehmannia glutinosa Libosch. (Zhang et al. 2008) 16
Figure 2. Elevated Plus-maze 20
Figure 3. Effect of a single treatment of the Rehmannia glutinosa on the percentage of the time spent in the elevated plus-maze over a 5-min test period in the mice. Each bar represents mean ± S.E.M of 8-10 mice. P values for the group... 30
Figure 4. Effect of a single treatment of the Rehmannia glutinosa on the percentage of the number of entries into the elevated plus-maze over a 5-min test period in the mice. Each bar represents mean ± S.E.M of 8-10 mice. P values for the... 31
Figure 5. Anxiolytic-like effects of Rehmannia glutinosa were blocked by flumazenil but not by WAY 100635. The data is expressed as the mean (± S.E.M.) of the percentage of the time spent in and the number of entries into the open arms of the elevated plus-maze, 1 h... 36
Figure 6. Performance of mice in the horizontal wire test 1h after oral administration of control, diazepam (5 mg/kg), or Rehmannia glutinosa. Data represent percentage of mice grasping the wire after administration of R. glutinosa. Each bar represents the mean ± S.E.M.... 38
Figure 7. The effect of Rehmannia glutinosa and diazepam on the locomotor activity in the open field test over 5 min test in mice. Each bar represents mean ± S.E.M obtained from 8-10 mice. P values for group comparisons were obtained by one way ANOVA followed by... 41
Figure 8. Anti-depressive effect of single treatment with Rehmannia glutinosa (ERG) on the forced swimming test in mice. ERG was orally treated 1 h prior to the test at designated dosage regimens. Imipramine was used as a positive control and... 44
Figure 9. Anti-depressive effect of single treatment with Rehmannia glutinosa (ERG) on the tail suspension test in mice. ERG was orally treated 1 h prior to the test at designated dosage regimens. Imipramine was used as a positive control and intraperitoneally treated... 47