표제지
목차
ABSTRACT 8
I. 서론 10
II. 재료 및 방법 11
1. Sweet BV의 조제 11
2. 실험동물 11
2.1. 실험동물 및 사육 환경 11
2.2. 군의 분리 12
2.3. 1회 시술 용량의 설정 및 시술 12
3. 관찰 및 검사 13
3.1. 일반증상 관찰 13
3.2. 체중측정 13
3.3. 부검 13
3.4. 조직병리학적 검사 13
4. 통계 처리 14
III. 결과 15
1. 일반증상 관찰 15
2. 체중변화 16
3. 부검 및 조직병리학적 검사 17
IV. 고찰 18
V. 결론 24
參考文獻 26
Table 1. Clinical signs in dose-escalating single intramuscular toxicity study of Beagle dogs 47
Table 2. Body weight changes in dose-escalating single intramuscular toxicity study of Beagle dogs 49
Table 3. Necropsy findings in dose-escalating single intramuscular toxicity study of Beagle dogs 51
Table 4. Histopathological findings in dose-escalating single intramuscular toxicity study of Beagle dogs 52
Fig. 1. Body weight changes of male and female in doseescalating single intramuscular toxicity study of Sweet Bee Venom in Beagle dogs. 32
Fig. 2. The tissue of left and right thigh muscle from single dose toxic test of Sweet Bee Venom in Beagle dogs. 34
Fig. 3. The tissue of lung and alveolar which showed abnormal changes from single dose toxic test of Sweet Bee Venom in Beagle dogs. 36
Fig. 4. The tissue of brain from single dose toxic test of Sweet Bee Venom in Beagle dogs. 38
Fig. 5. The tissue of liver from single dose toxic test of Sweet Bee Venom in Beagle dogs. 39
Fig. 6. The tissue of lung from single dose toxic test of Sweet Bee Venom in Beagle dogs. 40
Fig. 7. The tissue of kidney from single dose toxic test of Sweet Bee Venom in Beagle dogs. 41
Fig. 8. The tissue of spinal cord from single dose toxic test of Sweet Bee Venom in Beagle dogs. 42
Fig. 9. Most probable conformations of melittin. 43
Fig. 10. Molecular dynamics simulation of oligomeric melittin. 44
Fig. 11. HPLC analysis of Sweet BV. Sweet BV was consist of 99.9% pure melittin by HPLC analysis. 45