표제지
감사의 글
목차
I. 서론 11
II. 재료 및 방법 15
1. 실험 재료 15
1) 재료 15
2) 시료 추출 15
3) 시약 및 기기 17
2. 실험 방법 19
1) 항산화 효과 측정 19
2) 미백효과 측정 21
3) 세포 생존율 실험 22
4) 천연복합물을 첨가한 라멜라 액정 제조 23
4) 아토피 피부 개선 액정 리포좀 로션 제조 27
5) 아토피 피부 개선 로션의 임상시험 36
III. 결과 및 고찰 38
1. 항산화 효과 38
1) 전자공여능 측정결과 38
2) SOD 유사활성 측정결과 41
3) ABTS 라디칼 저해 활성 43
4) Hydrogen Peroxide 저해능 측정결과 45
2. 미백효과 측정 47
1) Tyrosinase 저해 활성 측정 47
3. 세포 생존율 측정 49
1) MTT assay에 의한 세포 생존율 측정 49
4. 천연복합물을 첨가한 라멜라 액정 제조 및 특성 51
1) 광학현미경 관찰 51
2) 라멜라 액정의 열분석 52
3) 라멜라 액정의 X-ray 회절분석 54
5. 아토피 피부 개선 액정 리포좀 로션 제조 56
1) 광학현미경 관찰 56
2) 전자투과현미경 (TEM) 측정 57
3) 라멜라 액정과 액정 리포좀 로션의 안정성 및 안전성 58
6. 아토피 피부 개선 로션의 임상 결과 70
1) 피부수분함유량 측정 70
2) 경표피수분손실량 측정 72
IV. 참고문헌 73
초록 79
Abstract 81
Table 1. The experimental formulation of lamellar liquid crystal containing herb complex 25
Table 2. The experimental formulation of liposome lotion containing herb complex 28
Table 3. Check points in stability test of liposome lotion containing herb complex in constant temperature conditions (0, 25, 40℃) 32
Table 4. The expression method of patch test 35
Table 5. Results of stability test of liposome lotion containing herb complex in constant temperature conditions (0℃, 25℃, 40℃) 63
Table 6. Results of Artificial Sun lamp test of liposome lotion containing herb complex. 65
Table 7. Results of Sun test of liposome lotion containing herb complex. 66
Table 8. Results of stability test of liposome lotion containing herb complex in cycle chamber condition. 68
Table 9. Results for patch test of liposome lotion containing herb complex. 69
Fig. 1. Procedure for extraction from herb complex. 16
Fig. 2. Preparation of lamellar liquid crystal containing herb complex. 24
Fig. 3. Preparation of liposome lotion containing herb complex. 27
Fig. 4. Procedure of viscosity measurement. 31
Fig. 5. Cycle temperature change with day for 1 cycle. 34
Fig. 6. Electron donating ability of herb complex. 40
Fig. 7. SOD-like activity of herb complex. 42
Fig. 8. ABTS radical scavenging activity of herb complex. 44
Fig. 9. Hydrogen peroxide scavenging activity of herb complex. 46
Fig. 10. Inhibition rate of herb complex on tyrosinase. 48
Fig. 11. Cell viability of herb complex on mouse macrophage Raw 264.7 cells. 50
Fig. 12. Photograph of lamellar liquid crystal by optical microscopy 51
Fig. 13. Phase transition temperature of lamellar liquid crystal containing herb complex by DSC. 53
Fig. 14. Crystallization pattern of lamellar liquid crystal containing herb complex by XRD. 55
Fig. 15. Photograph of liposome lotionby optical microscopy 56
Fig. 16. Photograph of liposome lotion by TEM 57
Fig. 17. pH change of liposome lotion containing herb complex. 59
Fig. 18. Viscosity change of the liposome lotion containing herb complex 61
Fig. 19. Moisturizing effect of liposome lotion on the skin. 71
Fig. 20. Transepidermal water loss values of liposome lotion on the skin. 72