표제지
목차
I. 서론 7
II. 재료 및 방법 11
1. 실험 재료 11
2. 추출물 제조 11
(1) 합환피 물 추출물(Albizziae Cortex Water extraction; ACW) 11
(2) 합환피 에탄올 추출물(Albizziae Cortex Ethanol extraction; ACE) 11
3. 시약 및 기기 13
4. 폴리페놀 화합물 함량 15
5. 플라보노이드 화합물 함량 15
6. Xanthine oxidase저해활성 16
7. SOD 유사활성능(Superoxide dismutase like activity) 17
8. 전자공여능(Electron donating ability: EDA) 18
9. 아질산염 소거능(Nitrite-scavenging ability: NSA) 19
10.통계처리 20
III. 결과 및 고찰 21
1. 합환피 추출물의 고형분 수율 21
2. 합환피 추출물의 폴리페놀 화합물 함량 23
3. 합환피 추출물의 플라보노이드 화합물 함량 26
4. 합환피 추출물의 xanthine oxidase저해활성 28
5. 합환피 추출물의 SOD 유사활성능 31
6. 합환피 추출물의 전자공여능 35
7. 합환피 추출물의 아질산염 소거능 38
IV. 결론 43
참고문헌 45
(국문초록) 57
(Abstract) 59
Table 1. Extraction yield of water and ethanol extracts from Albizziae Cortex 21
Table 2. Total polyphenol compound contents of water and ethanol extracts from Albizziae Cortex 24
Table 3. Total flavonoid compound contents of water and ethanol extracts from Albizziae Cortex 27
Table 4. Xanthine oxidase inhibition of water and ethanol extracts from Albizziae Cortex 29
Table 5. Superoxide dismutase (SOD)-like activities of water and ethanol extracts from Albizziae Cortex 33
Table 6. Electron donating abilities of water and ethanol extracts from Albizziae Cortex 36
Table 7. Nitrite scavenging abilities of water and ethanol extracts from Albizziae Cortex atvarious pH condition 40
Fig.1. Extraction procedure of the stem bark from Albizziae Cortex. 12
Fig.2. Procedure experiment of Albizziae Cortex extract. 14
Fig.3. Extraction yield of water and ethanol extracts from Albizziae Cortex. ACW : Albizziae Cortex Water extract, ACE : Albizziae Cortex Ethanol extract. 22
Fig.4. Total polyphenol compound contents of water and ethanol extracts from Albizziae Cortex. ACW : Albizziae Cortex Water extract, ACE : Albizziae Cortex Ethanol extract. All value presents the mean±SD of triplicate determinations. 25
Fig.5. Total flavonoid compound contents of water and ethanol extracts from Albizziae Cortex. ACW : Albizziae Cortex Water extract, ACE : Albizziae Cortex Ethanol extract. All value presents the mean±SD of triplicatede terminations. 27
Fig.6. Xanthine oxidase inhibition of water and ethanol extracts from Albizziae Cortex. ACW : Albizziae Cortex Water extract, ACE : Albizziae Cortex Ethanol extract, AsA : Ascorbic acid. All value presents the mean±SD of triplicate determinations, bar withi... 30
Fig.7. SOD-like activities of water and ethanol extracts from Albizziae Cortex. ACW : Albizziae Cortex Water extract, ACE : Albizziae Cortex Ethanol extract, AsA : Ascorbic acid. All value presents the mean±SD of triplicate determinations, bar within differen... 34
Fig.8. Electron donating abilities of water and ethanol extracts from Albizziae Cortex. ACW : Albizziae Cortex Water extract, ACE : Albizziae Cortex Ethanol extract, AsA : Ascorbic acid. All value presents the mean±SD of triplicate determinations, bar withi... 37
Fig.9. Nitrite scavenging abilities of water and ethanol extracts from Albizziae Cortex atpH 1.2 (a), 3.0 (b) and 6.0 (c) condition. ACW : Albizziae Cortex Water extract, ACE : Albizziae Cortex Ethanol extract, AsA : Ascorbic acid. All value presents the mean±SD of... 42