표제지
감사의 글
목차
I. 서론 12
II. 재료 및 방법 15
1. 실험 재료 15
1) 재료 15
2) 시료 추출 15
3) 시약 및 기기 17
2. 실험 방법 19
1) 항산화 효과 측정 19
2) 미백 효과 측정 22
3) 주름개선 효과 측정 23
4) 항균 효과 측정 24
5) 황백 추출물을 첨가한 W/O/W multiple emulsion제조 26
6) 황백 추출물을 첨가한 W/O/W multiple emulsion의 안정성 측정 29
7) 황백 추출물을 첨가한 W/O/W multiple emulsion의 유효성 측정 33
8) 황백 추출물을 첨가한 W/O/W multiple emulsion의 안전성 측정 34
III. 결과 및 고찰 35
1. 황백 추출물의 항산화 능 측정 결과 35
1) DPPH radical소거활성 확인 35
2) Superoxi dedismutase(SOD) 유사활성 확인 38
3) Xanthine oxidase 저해활성 확인 40
2. 미백 효과 측정 결과 42
1) Tyrosinase 저해 활성 확인 42
3. 주름개선 효과 측정 결과 45
1) Elastase 저해능 확인 45
4. 항균 효과 측정 결과 47
1) 생육 저해 환(clearzone) 확인 47
5. 황백 추출물을 첨가한 W/O/W multiple emulsion의 안정성 측정 53
1) 황백추출물을 첨가한 W/O/W multiple emulsion의 입자 안정성 확인 53
2) 황백 추출물이 첨가된 W/O/W emulsion의 pH 측정 결과 55
3) 황백 추출물이 첨가된 W/O/W emulsion의 점도측정 57
4) 황백 추출물이 첨가된 W/O/W emulsion의 온도 (Incubation)에 따른 안정성 측정결과 59
5) 황백 추출물이 첨가된 W/O/W emulsion의 온도순환(Cycle chamber) 에 따른 안정성 측정결과 61
6. 황백 추출물을 첨가한 W/O/W multiple emulsion의 항산화 효과 측정 결과 63
1) 황백 추출물을 첨가한 W/O/W multiple emulsion의 DPPH radical 소거 능 확인 63
2) Superoxide dismutase(SOD) 유사활성 확인 65
3) Xanthine oxidase저해능 확인 67
7. 황백 추출물을 첨가한 W/O/W multiple emulsion의 미백 효과 측정 결과 69
1) Mushroom 유래의 Tyrosinase저해능 측정 69
8. 황백 추출물을 첨가한 W/O/W multiple emulsion의 주름개선 효과 측정 결과 71
1) Elastase저해능 확인 71
9. 황백 추출물을 첨가한 화장품의 안전성 측정 73
1) 첩포시험 73
IV. 참고문헌 75
(초록) 84
(Abtract) 86
Table 1. The experimental formulation of the W/O/W multiple emulsion containing Phellodendron amurense Rupr extracts 27
Table 2. Check points in stability test of the W/O/W multiple emulsion containing Phellodendron amurense Rupr extractsin constant temperature condition (0,25,40℃) 31
Table 3. The expression method of patch test. 34
Table 4. Antimicrobi alactivity of Phellodendron amurense Rupr extracts on several microorganisms. (PAR-W1: Phellodendron amurense Rupr water extracts, PAR-E2: Phellodendron amurense Rupr ethanol extracts) 48
Table 5. Antimicrobi alactivity of Phellodendron amurense Rupr extracts on several microorganisms (PAR-A3: Phellodendron amurense Rupr acetone extracts). 51
Table 6. pH measurement of Phellodendron amurense Rupr. 55
Table 7. Result of stability test of the W/O/W multiple emulsion containing Phellodendron amurense Rupr acetone extract in constant temperature conditions. (0,25,40℃) 60
Table 8. Result of stability test of the W/O/W multiple emulsion containing Phellodendron amurense Rupr in cycle chamber condition. 62
Table 9. Result of patch test of the W/O/W multiple emulsion containing Phellodendron amurense Rupr extracts. 73
Fig. 1. The procedure for extraction from Phellodendron amurense Rupr. 16
Fig. 2. Preparation of the W/O/W multiple emulsion containing Phellodendron amurense Rupr extracts. 28
Fig. 3. Cycle temperature change with day for 1. cycle. (-15℃, -10℃, 0℃, -5℃, 0℃, 5℃, 10℃, 15℃, 25℃, 37.5℃, 40℃) 32
Fig. 4. Electron donating ability of from Phellodendron amurense Rupr extracts. 37
Fig. 5. SOD-like activity of Phellodendron amurense Rupr extracts. 39
Fig. 6. Inhibition rate of Phellodendron amurense Rupr extracts on xanthine oxidase. 41
Fig. 7. The melanogenic pathway. 43
Fig. 8. Inhibition rate of Phellodendron amurense Rupr extracts on tyrosinase. 44
Fig. 9. Inhibition rate of Phellodendron amurense Rupr extracts on elastase. 46
Fig. 10. Antimicrobial activity of Phellodendron amurense Rupr water extracts on several microorganisms. 49
Fig. 11. Antimicrobial activity of Phellodendron amurense Rupr ethanol extracts on several microorganisms 50
Fig. 12. Antimicrobial activity of Phellodendron amurense Rupr acetone extracts on several microorganisms. 52
Fig. 13. Microscopic aspect of the w/o/w multiple emulsion containing Phellodendron amurense Rupr extracts. (×1000) 54
Fig. 14. pH measurement of the W/O/W multiple emulsion containing Phellodendron amurense Rupr extracts. 56
Fig. 15. Viscosity change of the W/O/W multiple emulsion containing Phellodendron amurense Rupr extract. 58
Fig. 16. Electron donating ability of W/O/W multiple emulsion containing Phellodendro namurense Rupr extract 64
Fig. 17. SOD-like activity of W/O/W multiple emulsion containing Phellodendro namurense Rupr extracts. 66
Fig. 18. Inhibition rate of W/O/W multiple emulsion containing Phellodendron amurense Rupr extracts on xanthine oxidase. 68
Fig. 19. Inhibition rate of W/O/W multiple emulsion containing Phellodendron amurense Rupr extracts on tyrosinase. 70
Fig. 20. Inhibition rate of W/O/W multiple emulsion containing Phellodendron amurense Rupr extracts on elastase. 72
Fig. 21. PhotographofpatchtestoftheW/O/W multipleemulsion containing Phellodendron amurense Rupr extracts. 74