표제지
감사의 글
목차
I. 서론 11
II. 재료 및 방법 14
1. 재료 14
2. 복분자 균사체배양 14
3. 복분자와 복분자 균사체 유용성분 분석 15
1) 일반성분 분석 15
2) 유리당 정량 16
3) 비타민C 정량 17
4) pH, Brix 측정 18
5) 무기질 정량 18
4. 복분자와 복분자 균사체 추출물 제조 20
5. 복분자와 복분자 균사체의 항산화 효과 21
1) 총 폴리페놀 정량 21
2) 전자공여능(Electron donating ability)측정 21
3) Superoxide dismutase(SOD) 유사활성 측정 22
6. 복분자와 복분자 균사체 추출물의 암세포 성장 억제 효과 23
1) 실험 재료 23
2) 암세포 증식억제 효과 23
7. 통계처리 23
III. 결과 및 고찰 24
1. 일반성분 24
2. 유리당 정량 25
3. 비타민 C 함량 26
4. 당도 및 pH 측정 27
5. 무기질 함량 28
6. 복분자와 복분자 균사체의 항산화 효과 30
1) 총 폴리페놀 정량 30
2) 전자공여능(Electron donating ability) 31
3) Superoxide dismutase(SOD) 유사활성 33
7. 복분자와 복분자 균사체의 암세포 성장억제 효과 35
1) 유방암 세포(MCF-7 cell)에 미치는 영향 35
2) 자궁경부암 세포(Hela cell)에 미치는 영향 42
IV. 참고문헌 49
(초록) 55
(Abstract) 57
Table 1. Analytical conditions of HPLC for the determination of free sugars 16
Table 2. Analytical conditions of HPLC for the ascorbic acid 17
Table 3. Operation conditions of ICP analysis 19
Table 4. Contents of general component in the Rubus coreanus miquel and Lentinus edodes mycelia cultured with Rubus coreanus miquel 24
Table 5. Contents of free sugar in the Rubus coreanus miquel and Lentinus edodes mycelia cultured with Rubus coreanus miquel 25
Table 6. Contents of vitamin C in the Rubus coreanus miquel and Lentinus edodes mycelia cultured with Rubus coreanus miquel 26
Table 7. Brix and pH of the Rubus coreanus miquel and Lentinus edodes mycelia cultured with Rubus coreanus miquel 27
Table 8. Contents of mineral in the Rubus coreanus miquel and Lentinus edodes mycelia cultured with Rubus coreanus miquel 29
Table 9. Contents of polyphenol in the Rubus coreanus miquel and Lentinus edodes mycelia cultured with Rubus coreanus miquel 30
Fig. 1. Electron donating ability of Rubus coreanus miquel and Lentinus edodes mycelia cultured with Rubus coreanus miquel. 32
Fig. 2. SOD like activity of Rubus coreanus miquel and Lentinus edodes mycelia cultured with Rubus coreanus miquel. 34
Fig. 3. Morphology of MCF-7 cells treated with water extract from Rubus coreanus miquel. 36
Fig. 4. Morphology of MCF-7 cells treated with 70% EtOH extract from Rubus coreanus miquel. 37
Fig. 5. Morphology of MCF-7 cells treated with water extract from Lentinus edodes mycelia cultured with Rubus coreanus miquel. 38
Fig. 6. Morphology of MCF-7 cells treated with 70% EtOH extract from Lentinus edodes mycelia cultured with Rubus coreanus miquel. 39
Fig. 7. Growth inhibiton of MCF-7 cells by extracts from Rubus coreanus miquel and Lentinus edodes mycelia cultured with Rubus coreanus miquel. 41
Fig. 8. Morphology of Hela cells treated with water extract from Rubus coreanus miquel. 43
Fig. 9. Morphology of Hela cells treated with 70% EtOH extract from Rubus coreanus miquel. 44
Fig. 10. Morphology of Hela cells treated with water extract from Lentinus edodes mycelia cultured with Rubus coreanus miquel. 45
Fig. 11. Morphology of Hela cells treated with 70% EtOH extract from Lentinus edodes mycelia cultured with Rubus coreanus miquel. 46
Fig. 12. Growth inhibiton of Hela cells by extracts from Rubus coreanus miquel and Lentinus edodes mycelia cultured with Rubus coreanus miquel. 48