표제지
목차
I. 서론 7
II. 재료 및 방법 9
1. 실험 재료 9
(1)환류 물 추출물(WE : Reflux Water extraction) 9
(2)환류 에탄올 추출물(EE : Reflux Ethanol extraction) 9
3. 시약 및 기기 11
4. 플라보노이드 화합물 함량 12
5. 폴리페놀 화합물 함량 12
6. 전자공여능(Electron donating ability : EDA) 13
7. SOD 유사활성능(Superoxide dismutase like activity) 14
8. 아질산염 소거능(Nitrite-scavenging ability : NSA) 15
9. Xanthine oxidase저해 16
10. 통계처리 17
III. 결과 및 고찰 18
1. 토아산의 추출 수율 18
2. 추출물의 플라보노이드 화합물 함량 20
3. 추출물의 폴리페놀 화합물 함량 22
4. 전자공여능 24
5. SOD 유사활성능 27
6. 아질산염 소거능 31
7. Xanthine oxidase저해활성 35
IV. 결론 38
참고문헌 40
(국문초록) 50
(Abstract) 52
Table 1. Extraction yield of water and ethanol extracts from S. palmata aerial part 18
Table 2. Total flavonoid compound contents of water and ethanol extracts from S. palma taaerial part 20
Table 3. Total polyphenol compound contents of water and ethanol extracts from S. palma taaerial part 23
Table 4. Electron donating abilities of water and ethanol extracts from S. palmata aerial part 25
Table 5. Superoxi dedismutase (SOD)-like activities of water and ethanol extracts from S. palmata aerial part 29
Table 6. Nitrite scavenging abilities of water and ethanol extracts from Syneilesis palmata aerial part at various pH condition. 33
Table 7. Xanthine oxidase inhibition of water and ethanol extracts from S. palmata aerial part 36
Fig.1. Extraction procedure of theaerialpartsfrom S. palmata. 10
Fig.2. Extraction yield of water and ethanol extracts from S. palmata aerial part. WE : Water extract, EE : Ethanol extract. 19
Fig.3. Total flavonoid compound contents of water and ethanol extracts from S. palmata aerial part. WE : Water extract, EE :Ethanol extract. All value presents the mean±SD of triplicate determinations. 21
Fig.4. Total polyphenol compound contents of water and ethanol extracts from S. palmata aerial part. WE : Water extract, EE : Ethanol extract. All value presents the mean±SD of triplicate determinations. 23
Fig.5. Electron donating abilities of water and ethanol extracts from S. palmata aerial part. WE : Water extract, EE : Ethanol extract, AsA : Ascorbic acid. All value presents the mean±SD of triplicate determinations, bar within different letters are significantly different at p<0.05 by Duncan's multiple range test 26
Fig.6. SOD-like activities of water and ethanol extracts from S. palmata aerial part. WE : Water extract, EE : Ethanol extract, AsA : Ascorbic acid. All value presents the mean±SD of triplicate determinations, bar within different letters are significantly different at p<0.05 by Duncan's multiple range test 30
Fig.7. Nitrite scavenging abilities of water and ethanol extracts from S. palmata aerial part at pH 1.2 (a) and 3.0 (b) .WE : Water extract, EE : Ethanol extract, AsA : Ascorbic acid. All value presents the mean ±SD of triplicate determinations, bar within different letters are significantly different at p<0.05 by Duncan's multiple range test. 34
Fig.8. Xanthine oxidase inhibition of water and ethanol extracts from S. palmata aerial part. WE : Water extract, EE : Ethanol extract, AsA : Ascorbic acid. All value presents the mean±SD of triplicate determinations, bar within different letters are significantly different at p<0.05 by Duncan's multiple range test. 37