Title page
Contents
Ⅰ. INTRODUCTION 8
Ⅱ. MATERIALS & METHODS 13
Ⅱ.1. Animals and Husbandry 13
Ⅱ.2. Test article and Formulation 13
Ⅱ.3. Induction of Subacute Hepatic damage 14
Ⅱ.4. Measurement of Body Weights 15
Ⅱ.5. Measurement of Liver Weights 15
Ⅱ.6. Measurement of AST and ALT levels 16
Ⅱ.7. Assays for hepatic contents 16
Ⅱ.8. Statistical Analyses 17
Ⅲ. RESULTS 19
Ⅲ.1. Changes on the Body Weights and Gains 19
Ⅲ.2. Changes on the Liver Weight 20
Ⅲ.3. Changes of Serum AST Levels 22
Ⅲ.4. Changes of Serum ALT Levels 24
Ⅲ.5. Changes of Liver Protein Contents 26
Ⅲ.6. Changes of Liver Malondialdehyde Contents 27
Ⅲ.7. Changes of Liver Hydroxyproline Contents 28
Ⅳ. DISCUSSION 49
Ⅴ. SUMMARY 59
Ⅵ. REFERENCES 63
초록 74
Table 1. Group ID and Composition of Test Articles Used in This Study. 31
Table 2. Changes on the Body Weight and Gains in intact and CCl₄-induced Subacute Hepatic Damaged Rats 32
Table 3. Changes on the Liver Weights in Intact and CCl₄-induced Subacute Hepatic Damaged Rats 33
Table 4. Changes on the Serum AST Levels in Intact and CCl₄-induced Subacute Hepatic Damaged Rats 34
Table 5. Changes on the Serum ALT Levels in Intact and CCl₄-induced Subacute Hepatic Damaged Rats 35
Table 6. Changes on the Liver protein, Malondialdehyde and Hydroxyproline Contents in Intact and CCl₄-induced Subacute Hepatic Damaged Rats 36
Fig 1. Body Weight Changes in Intact and CCl₄-induced Subacute Hepatic Damaged Rats after AC:PR 1:1 administration. Note that dramatical decreases of body weight were detected in all CCl₄-treated groups as compared with intact control from 7 days after CCl₄ treatment, respectively.... 38
Fig 2. Body Weight Changes in Intact and CCl₄-induced Subacute Hepatic Damaged Rats after AC:PR 1:2 administration. Note that dramatical decreases of body weight were detected in all CCl₄-treated groups as compared with intact control from 7 days after CCl₄ treatment, respectively.... 39
Fig 3. Body Weight Changes in Intact and CCl₄-induced Subacute Hepatic Damaged Rats after AC:PR 1:4 administration. Note that dramatical decreases of body weight were detected in all CCl₄-treated groups as compared with intact control from 7 days after CCl₄ treatment, respectively.... 40
Fig 4. Body Weight Changes in Intact and CCl₄-induced Subacute Hepatic Damaged Rats after AC:PR 1:6 administration. Note that dramatical decreases of body weight were detected in all CCl₄-treated groups as compared with intact control from 7 days after CCl₄ treatment, respectively.... 41
Fig 5. Body Weight Changes in Intact and CCl₄-induced Subacute Hepatic Damaged Rats after AC:PR 1:8 administration. Note that dramatical decreases of body weight were detected in all CCl₄-treated groups as compared with intact control from 7 days after CCl₄ treatment, respectively.... 42
Fig 6. Body Weight Changes in Intact and CCl₄-induced Subacute Hepatic Damaged Rats after AC:PR 1:10 administration. Note that dramatical decreases of body weight were detected in all CCl₄-treated groups as compared with intact control from 7 days after CCl₄ treatment, respectively.... 43
Fig 7. Body Weight Changes in Intact and CCl₄-induced Subacute Hepatic Damaged Rats after AC:PR 2:1 administration. Note that dramatical decreases of body weight were detected in all CCl₄-treated groups as compared with intact control from 7 days after CCl₄ treatment, respectively.... 44
Fig 8. Body Weight Changes in Intact and CCl₄-induced Subacute Hepatic Damaged Rats after AC:PR 4:1 administration. Note that dramatical decreases of body weight were detected in all CCl₄-treated groups as compared with intact control from 7 days after CCl₄ treatment, respectively.... 45
Fig 9. Body Weight Changes in Intact and CCl₄-induced Subacute Hepatic Damaged Rats after AC:PR 6:1 administration. Note that dramatical decreases of body weight were detected in all CCl₄-treated groups as compared with intact control from 7 days after CCl₄ treatment, respectively.... 46
Fig 10. Body Weight Changes in Intact and CCl₄-induced Subacute Hepatic Damaged Rats after AC:PR 8:1 administration. Note that dramatical decreases of body weight were detected in all CCl₄-treated groups as compared with intact control from 7 days after CCl₄ treatment, respectively.... 47
Fig 11. Body Weight Changes in Intact and CCl₄-induced Subacute Hepatic Damaged Rats after AC:PR 10:1 administration. Note that dramatical decreases of body weight were detected in all CCl₄-treated groups as compared with intact control from 7 days after CCl₄ treatment, respectively.... 48
EQUATION 1. Body Weigh Gains (g) = Body weight gains throughout the whole experimental periods (Day 0 ~ Day 70) 15
EQUATION 2. Relative liver weight (%) = [(Absolute liver weight / Body weight at sacrifice) × 100] 15
EQUATION 3. Changes of serum AST and ALT levels (IU/L) = Serum levels at sacrifice - Serum levels at 1 day before dosing 16
EQUATION 4. Percentage Changes vs intact control (%) = ((Data of CCl₄ control - Data of intact control)/Data of intact control) × 100 18
EQUATION 5. Percentage Changes vs CCl₄ control (%) = ((Data of test article-dosing groups - Data of CCl₄ control)/Data of CCl₄ control) × 100 18