Title page
ACKNOWLEDGEMENT
Contents
ABSTRACT 7
Ⅰ. Introduction 8
Ⅱ. MARERIALS AND METHODS 10
Ⅱ-1. Cell Culture 10
Ⅱ-2. Calculation of nitrogen to phosphorus (N/ P) ratio 10
Ⅱ-3. Transfection Procedures 10
Ⅱ-4. Transfection Efficiency Measurements 11
Ⅱ-5. Statistical Analysis 12
Ⅲ. RESULTS 13
Ⅲ-1. Transfection efficiency and cytotoxicity of 25kDa L-PEI at varying N/ P ratio in different cell lines to determine optimal N/ P ratio: 13
Ⅲ-2. Determination of optimal DNA concentration complexed with the 25 kDa L-PEI at N/ P ratio 40 in different cell lines: 13
Ⅲ-3. Compatibility of transfection efficiency and cytotoxicity of 25 kDa L-PEI with three different non-viral vectors in different cell lines: 14
Ⅳ. Discussion 16
Ⅴ. REFERENCES 23
Summary in Korean 27
Figure 1. Transfection efficiency and cytotoxicity of 25 kDa L-PEI at varying N/P ratio in different cell lines to determine optimal N/P ratio. pEGFP-C1 plasmid DNA (2 ㎍) was complexed with the desired amount of 25 kDa L-PEI to obtain a chosen N/P ratio from 10 to 40. Transfection efficiency and toxicity were measured by flow... 19
Figure 2. Determination of optimal amounts of DNAs complexed with the 25 kDa L-PEI at N/P ratio 40 in different cell lines. The pEGFP plasmid DNA ranging from 1 to 3 ㎍ was complexed with the amount of L-PEI at N/P ratio 40. Transfection efficiency and toxicity were measured by flow cytometry, followed by PI staining... 20
Figure 3. Compatibility of Transfection efficiency and cytotoxicity of L-PEI with three different transfection reagents in different cell lines. Transfection efficiency and toxicity were measured by flow cytometry, followed by PI staining. The means ±SEM of four independent experiments are indicated. The optimal DNA concentration and... 21