title page
Contents
List of abbreviations 8
(Abstract) 9
Introduction 11
Materials and methods 14
I. Materials 14
1. Plasmid constructions 14
2. Antibodies 14
II. Methods 14
1. Cell culture, transfection and CHX treatment 14
2. Immunoprecipitation and Immunoblotting 15
3. Kinase assay 16
4. Fluorescent microscopy 16
Results 17
1. The interaction of δ-catenin with p190RhoGEF depends on phophorylation of its Thr-454 residue by Akt1, which is critical for δ-catenin-indued dendrite-like process formation in NIH 3T3 fibroblasts 17
2. The interaction of δ-catenin with 14-3-3ε/ζ increases its stability, but the binding domain is isoform-specific, and Thr-454 residue on δ-catenin by Akt1 is not required for the effects of 14-3-3ε/ζ on δ-catenin(이미지참조) 25
3. The interaction of δ-catenin with 14-3-3 is important for the dendritic branches 29
Discussion 31
Reference 36
(국문초록) 40
Acknowledgements 41
Figure 1. The interaction of δ-catenin with p190RhoGEF depends on the phosphorylation of its Thr-454 residue by Akt1 21
Figure 2. The interaction of δ-catenin with p190RhoGEF increased its sstability 23
Figure 3. Effects of wild type and mutant δ-catenin on the dendritic-like process formation in NIH 3T3 fibroblasts on the dendrogenesis 24
Figure 4. The interaction of δ-catenin with 14-3-3ε or 14-3-3ζ occurs through different binding domain, which increases its stability 27
Figure 5. Effects of treatment with sc138, a specific inhibitor peptide of 14-3-3 interaction with its substrate, on the dendritic-like process formation of NIH 3T3 fibroblasts 30