Title page
Acknowledgement
Contents
Abstract 9
I. Introduction 10
II. Materials and Methods 18
II. 1. Isolation and culture of MSC from UCB and BM 18
II. 2. Total RNA extraction 19
II. 3. Microarray experiments 19
II. 4. Microarray data analysis 20
II. 5. Annotation discovery 21
III. Results 23
III. 1. Reproducibility and sensitivity of microarray experiments 23
III. 2. Gene expression patterns of CB-MSC and BM-MSC 26
III. 3. Differentially expressed genes in each population 28
Discussion 37
References 41
Table 1. Number of probes according to the mean of signal value and ratio. 29
Table 2. Highly expressed genes in CB-MSC. 31
Table 3. Highly expressed genes in BM-MSC. 34
Table 4. Functional classification of highly expressed genes by Panther Biological Process 36
Figure 1. Matrix of scatter plots of the normalized log signal values and correlation coefficients between any two of the microarray data sets. Numbers in the matrix are correlation 25
Figure 2. Differentilly expressed genes in CB-MSC and BM-MSC. 27