표제지
감사의 글
목차
I. 서론 10
II. 재료 및 방법 14
1. 실험 재료 14
1) 재료 14
2) 시료 추출 15
3) 시약 및 기기 17
2. 실험 방법 18
1) 항산화 효과 측정 18
(1) 전자공여능 측정 18
(2) Superoxide dismutase(SOD) 유사활성 측정 19
(3) Xanthine oxidase 저해활성 측정 20
2) 미백 효과 측정 21
(1) Tyrosinase 저해 활성 측정 21
3) 십미패독탕 W/O/W 다중에멀젼의 제조 처방 및 방법 22
4) 십미패독탕을 첨가한 W/O/W 다중에멀젼의 항산화 효과 측정 24
(1) 십미패독탕을 첨가한 W/O/W 다중에멀젼의 전자공여능 측정 24
(2) 십미패독탕을 첨가한 W/O/W 다중에멀젼의 Superoxide dismutase(SOD) 유사활성 측정 24
(3) 십미패독탕을 첨가한 W/O/W 다중에멀젼의 Xanthine oxidase 저해 활성 측정 25
5) 십미패독탕을 첨가한 W/O/W 다중에멀젼의 미백 효과 측정 26
(1) 십미패독탕을 첨가한 W/O/W 다중에멀젼의 Tyrosinase 저해 활성 측정 26
6) 통계처리 26
7) 십미패독탕 W/O/W 다중에멀젼의 안정성 실험 27
(1) pH 측정 27
(2) 점도 측정 28
(3) 일반적 보존 시험 29
(4) 특수ㆍ가혹 보존 시험 30
III. 결과 및 고찰 31
1. 항산화능 측정 31
1) 전자공여능 확인 31
2) SOD 유사활성 검증 33
3) Xanthine oxidase 저해 활성 확인 35
2. 미백효과 측정 37
1) Tyrosinase 저해 활성 확인 37
3. 십미패독탕이 첨가된 W/O/W 다중에멀젼의 제조 40
4. 십미패독탕을 첨가한 W/O/W 다중에멀젼의 항산화능 측정 42
1) 십미패독탕을 첨가한 W/O/W 다중에멀젼의 전자공여능 확인 42
2) 십미패독탕을 첨가한 W/O/W 다중에멀젼의 SOD 유사활성 확인 44
3) 십미패독탕을 첨가한 W/O/W 다중에멀젼의 xanthine oxidase 저해 활성 확인 46
5. 십미패독탕을 첨가한 W/O/W 다중에멀젼의 미백 효과 측정 48
1) 십미패독탕을 첨가한 W/O/W 다중에멀젼의 tyrosinase 저해 활성 확인 48
6. 십미패독탕이 첨가된 W/O/W 다중에멀젼의 안정성 확인 50
1) 십미패독탕이 첨가된 W/O/W 다중에멀젼의 pH 측정 50
2) 십미패독탕이 첨가된 W/O/W 다중에멀젼의 점도 측정 52
3) 일반적 보존 시험 54
(1) 온도 (Incubation)에 따른 안정성 54
(2) 광안정성 56
4) 특수ㆍ가혹 보존 시험 59
(1) 온도순환(Cycle chamber)에 따른 안정성 59
(2) 냉ㆍ해동순환(Freeze & Thaw cycling)에 따른 안정성 61
IV. 참고문헌 63
초록 70
Abstract 72
Table 1. Composition of Sibmipaedogtang (十味敗毒湯) 14
Table 2. The experimental formulation of the W/O/W multiple emulsion containing Sibmipaedogtang 22
Table 3. Results of stability test of the W/O/W multiple emulsion containing Sibmipaedogtang in constant temperature conditions (0℃, 25℃, 40℃) 55
Table 4. Results of artificial sun lamp test of the W/O/W multiple emulsion containing Sibmipaedogtang. 57
Table 5. Results of sun test the W/O/W multiple emulsion containing Sibmipaedogtang 58
Table 6. Results of stability test of the W/O/W multiple emulsion containing Sibmipaedogtang in cycle chamber condition 60
Table 7. Results of stability test of the W/O/W multiple emulsion containing Sibmipaedogtang in freeze & thaw cycling condition 62
Fig. 1. The procedure for extraction from oriental medicine prescription-Sibmipaedogtang. 16
Fig. 2. The method of electron donating ability. 18
Fig. 3. The method of superoxide dismutase(SOD)-like activity. 19
Fig. 4. The method of xanthine oxidase inhibition 20
Fig. 5. The method of tyrosinase inhibition. 21
Fig. 6. Preparation of the W/O/W multiple emulsion containing Sibmipaedogtang. 23
Fig. 7. Procedure of viscosity measurement 28
Fig. 8. Electron donating ability of Sibmipaedogtang. SPW : Sibmipaedogtang water extract. SPE : Sibmipaedogtang ethanol extract. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 32
Fig. 9. SOD-like activity of Sibmipaedogtang. SPW : Sibmipaedogtang water extract. SPE : Sibmipaedogtang ethanol extract. Values are means of 3 replicates and those with different alphabet letters are significantly different at p <0.05. 34
Fig. 10. Inhibition rate of Sibmipaedogtang on xanthine oxidase. SPW : Sibmipaedogtang water extract. SPE : Sibmipaedogtang ethanol extract. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 36
Fig. 11. The melanogenic pathway. 38
Fig. 12. Inhibition rate of Sibmipaedogtang on tyrosinase oxidase. SPW : Sibmipaedogtang water extract. SPE : Sibmipaedogtang ethanol extract. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 39
Fig. 13. Photograph of the W/O/W multiple emulsion 40
Fig. 14. Microscopic aspect of the W/O/W multiple emulsion containing Sibmipaedogtang.( 1000). 41
Fig. 15. Electron donating ability of the W/O/W multiple emulsion containing Sibmipaedogtang. NME : Nomal W/O/W multiple emulsion. SPME : W/O/W multiple emulsion containing Sibmipaedogtang. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05 43
Fig. 16. SOD-like activity of the W/O/W multiple emulsion containing Sibmipaedogtang. NME : Nomal W/O/W multiple emulsion. SPME : W/O/W multiple emulsion containing Sibmipaedogtang. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 45
Fig. 17. Inhibition rate of the W/O/W multiple emulsion containing Sibmipaedogtang on xanthine oxidase. NME : Nomal W/O/W multiple emulsion. SPME : W/O/W multiple emulsion containing Sibmipaedogtang. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 47
Fig. 18. Inhibition rate of the W/O/W multiple emulsion containing Sibmipaedogtang on tyrosinase oxidase. NME : Nomal W/O/W multiple emulsion. SPME : W/O/W multiple emulsion containing Sibmipaedogtang. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 49
Fig. 19. pH change of the W/O/W multiple emulsion containing Sibmipaedogtang. 51
Fig. 20. Viscosity change of the W/O/W multiple emulsion containing Sibmipaedogtang. 53