표제지
목차
제1장 서론 8
제2장 재료 및 방법 14
1. 숙지황의 제조 14
2. 숙지황의 시료 채취 14
3. 중금속의 분석 16
1) 시료의 전처리 16
2) 시료의 분석기기 16
4. 농약의 분석 17
1) 시료의 전처리 17
2) 시료의 정제 18
3) 시료의 분석기기 18
제3장 결과 및 고찰 20
1. 숙지황 중 중금속의 안전성 평가 20
1) 지황 중 중금속 20
2) 숙지황 제조과정 중 중금속 21
3) 숙지황 중 중금속 23
2. 숙지황 중 농약의 안전성 평가 27
1) 컬럼의 분리능 27
2) 최소 검출량과 검출농도 33
3) 숙지황 중 잔류 농약 35
참고문헌 39
국문초록 41
Abstract 43
감사의 글 45
Table 1. Production of Rehmannia glutinosa on Korea 10
Table 2. Production of Rehmannia glutinosa in 2001 on Korea 10
Table 3. Health advisory level for toxic heavy metals in herbal materials. 12
Table 4. Operating conditions of ICP-MS for analysis of As, Cd and Pb. 17
Table 5. Group for analysis of pesticides in Rehmannia glutinosa on the gas chromatography-ECD. 19
Table 6. Heavy metals in rice wine, dried Rehmannia glutinosa and Rehmanniae Radix Preparata. 21
Table 7. Heavy metals in Rehmanniae Radix Preparata on Korea. 25
Table 8. Heavy metals in Rehmanniae Radix Preparata on China. 25
Table 9. Resolution of pesticides on the gas chromatography-ECD. 33
Table 10. Detection limit and qualified detection concentration on the gas chromatography-ECD for evaluation of residual pesticides. 34
Table 11. Residual levels of pesticides in rice wine, dried Rehmannia glutinosa and Rehmanniae Radix Preparata. 36
Table 12. Residual levels of pesticides in Rehmanniae Radix Preparata on Korea. 37
Table 13. Residual levels of pesticides in Rehmanniae Radix Preparata on China. 38
Fig. 1. Rehmannia glutinosa and Rehmanniae Radix Preparata. 8
Fig. 2. Steaming equipment, thermal drying equipment and sunshine drying room for manufacturing process of Rehmanniae Radix Preparata. 15
Fig. 3. Standard curve of heavy metals for As(△), Cd(○) and Pb(●) on ICP-MS. 20
Fig. 4. Heavy metals for As on manufacturing process of Rehmanniae Radix Preparata. 22
Fig. 5. Heavy metals for Cd on manufacturing process of Rehmanniae Radix Preparata 22
Fig. 6. Heavy metals for Pb on manufacturing process of Rehmanniae Radix Preparata 23
Fig. 7. Compared with Korea Food & Drug Administration(KFDA) advisory level with heavy metals in Rehmanniae Radix Preparata. 26
Fig. 8. GC-ECD chromatogram of standard pesticides for 1. BHC-δ, 2. BHC-β, 3. Fenitrothion, 4. Penthoate, 5. Endosulfan-α, 6. Dieldrin, 7. Endosulfan-β and 8. Endosulfan-sulfate 29
Fig. 9. GC-ECD chromatogram of standard pesticides for 1. BHC-γ, 2. Aldrin, 3. DDD, 4. DDT-p,p, 5. Permethrin and 6. Fenvalerate such as 0.1ng. 30
Fig. 10. GC-ECD chromatogram of standard pesticides for 1. BHC-α, 2. Chlorpyrifos, 3. Tolyfluanid, 4. Captan and 5. DDT-o,p such as 0.1ng. 31
Fig. 11. GC-ECD chromatogram of standard pesticides for 1. Quintozene, 2. Vinclozolin, 3. DDE and 4. Chlorfenapyr such as 0.1ng. 32