표제지
감사의 글
목차
I. 서론 10
II. 재료 및 방법 13
1. 실험재료 13
1) 시료 제조 13
2) 시약 및 기기 15
(1) 항산화능 측정 시약 15
(2) 미백효과 측정 시약 15
(3) 실험에 사용된 기기 15
2. 실험 방법 16
1) 폴리페놀 W/O/W 다중에멀젼의 제조 처방 및 방법 16
2) 항산화 효과 측정 19
(1) 전자공여능 측정 19
(2) Superoxide dismutase (SOD) 유사활성 측정 20
(3) Xanthine oxidase 저해활성 측정 21
3) 미백 효과 측정 22
(1) Tyrosinase 저해 활성 측정 22
4) 폴리페놀 w/o/w 다중에멀젼의 안정성 실험 23
(1) pH 측정 23
(2) 점도 측정 24
(3) 일반적 보존 시험 25
(4) 특수ㆍ가혹 보존 시험 27
III. 결과 및 고찰 28
1. 항산화능 측정 28
1) 전자공여능 확인 28
2) SOD 유사활성 확인 32
3) Xanthine oxidase 저해 활성 확인 36
2. 미백효과 측정 39
1) Tyrosinase 저해 활성 확인 39
3. 폴리페놀 W/O/W 다중에멀젼의 안정성 측정 44
1) 폴리페놀 W/O/W 다중에멀젼의 제조 44
2) 폴리페놀 W/O/W 다중에멀젼의 pH 측정 47
3) 폴리페놀 W/O/W 다중에멀젼의 점도 측정 49
4) 일반적 보존 시험 51
(1) 온도 (Incubation)에 따른 안정성 51
(2) 광안정성 53
5) 특수ㆍ가혹 보존 시험 56
(1) 온도순환 (Cycle chamber)에 따른 안정성 56
(2) 냉ㆍ해동순환 (Freeze & Thaw cycling)에 따른 안정성 58
IV. 참고문헌 60
초록 69
Abstract 70
Table 1. Preparation process of W/O/W multiple emulsion by two-step emulsification. 17
Table 2. Check points in stability test of the w/o/w cream containing polyphenol in constant temperature conditions (0, 25, 40℃) 25
Table 3. Results of stability test of the W/O/W multiple emulsion containing polyphenol in constant temperature conditions (0℃, 25℃, 40℃). 52
Table 4. Results of artificial sun lamp test of the W/O/W multiple emulsion containing polyphenol. 54
Table 5. Results of sun test of the W/O/W multiple emulsion containing polyphenol. 55
Table 6. Results of stability test of the W/O/W multiple emulsion containing poplyphenol in cycle chamber condition. 57
Table 7. Result of stability test in freeze & thaw cycling condition. 59
Fig. 1. Purification procedure for the polyphenol isolated from green tea. 14
Fig. 2. Preparation of polyphenol W/O/W multiple emulsion. 18
Fig. 3. The method of electron donating ability. 19
Fig. 4. The method of superoxide dismutase (SOD)-like activity. 20
Fig. 5. The method of xanthine oxidase inhibition. 21
Fig. 6. The method of tyrosinase inhibition measurement. 22
Fig. 7. Procedure of viscosity measurement. 24
Fig. 8. Cycle temperature change with day for 1 cycle. (-15℃, -10℃, -5℃, 0℃, 5℃, 10℃, 15℃, 25℃, 37.5℃, 40℃) 27
Fig. 9. Electron donating ability of polyphenol. PP : polyphenol. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 30
Fig. 10. Electron donating ability of polyphenol W/O/W multiple emulsion. NME : normal W/O/W multiple emulsion, PME : Polyphenol W/O/W multiple emulsion. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 31
Fig. 11. SOD-like activity of polyphenol. PP : polyphenol. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 34
Fig. 12. SOD-like activity of polyphenol W/O/W multiple emulsion. NME : normal W/O/W multiple emulsion, PME : polyphenol W/O/W multiple emulsion. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 35
Fig. 13. Inhibition rate of polyphenol on xanthine oxidase. PP : polyphenol. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 37
Fig. 14. Inhibition rate of polyphenol W/O/W multiple emulsion on xanthine oxidase. NME : normal W/O/W multiple emulsion, PME : polyphenol W/O/W multiple emulsion. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 38
Fig. 15. The melanogenic pathway. 41
Fig. 16. Inhibition rate of polyphenol on tyrosinase. PP : polyphenol. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 42
Fig. 17. Inhibition rate of polyphenol W/O/W multiple emulsion on tyrosinase. NME : normal W/O/W multiple emulsion, PME : polyphenol W/O/W multiple emulsion. Values are means of 3 replicates and those with different alphabet letters are significantly different at p<0.05. 43
Fig. 18. Photograph of the W/O/W multiple emulsion containing poiyphenol. A : normal W/O/W multiple emulsion, B : polyphenol W/O/W multiple emulsion. 45
Fig. 19. Microphotograph of polyphenol W/O/W multiple emulsion. 46
Fig. 20. pH change with concentration of polyphenol W/O/W mutiple emulsion. NME : normal W/O/W multiple emulsion, PME : polyphenol W/O/W multiple emulsion. 48
Fig. 21. Viscosity change of polyphenol W/O/W multiple emulsion. NME : normal W/O/W multiple emulsion, PME : polyphenol W/O/W multiple emulsion. 50