영문목차
Title page=0,1,4
Contents=i,5,3
List of Tables=iv,8,1
List of Figures=v,9,2
Abstract=vii,11,2
1. Introduction=1,13,1
1.1 Methane Oxidation=1,13,1
1.2 Aims of This Study=2,14,1
2. Theoretical Backgrounds=3,15,1
2.1 Methane as Greenhouse Gas=3,15,2
2.2 Methane Oxidation in Forest Soils=5,17,3
2.3 Controlling Variables for Methane Oxidation in soils=8,20,1
2.3.1 Temperature, moisture, organic matter and pH=8,20,2
2.3.2 Inorganic nitrogen=9,21,4
2.4 Methanotrophic Bacteria=13,25,1
2.4.1 Methanotrophic bacteria=13,25,2
2.4.2 Classification of Methanotrophic Bacteria=15,27,2
3. Materials&Methods=17,29,1
3.1 Site Description=17,29,1
3.2 Soil Sampling and Analysis=18,30,1
3.1.1 Soil sampling=18,30,1
3.2.2 Soil characteristics=19,31,1
3.2.3 Extracellular enzyme activity=19,31,1
3.3 Methane Oxidation Rates=20,32,1
3.3.1 Determination of methane oxidation rates=20,32,2
3.3.2 The effects of nitrogen input=22,34,2
3.4 Community Structure of Methanotrophic Bacteria=24,36,1
3.4.1 DNA extraction and PCR amplification=24,36,1
3.4.2 T-RFLP analysis=25,37,1
3.5 Statistical Analysis=26,38,1
4. Results=27,39,1
4.1 Soil Characteristics=27,39,1
4.1.1 Seasonal variation of soil characteristics=27,39,2
4.1.2 Site variations=28,40,2
4.2 Methane Oxidation Rates=30,42,1
4.2.1 in situ oxidation rates=30,42,3
4.2.2 Potential oxidation rates=33,45,3
4.2.3 The relationship between in situ rates and potential rates=36,48,2
4.2.4 Effects of nitrogen input=38,50,2
4.3 Community Structure of Methanotrophic Bacteria=40,52,1
4.3.1 PCR amplification=40,52,1
4.3.2 Distribution of type I methanotrophs=41,53,2
4.3.3 Distribution of type II methanotrophs=43,55,3
4.3.4 Relation of oxidation rates and community structure of methanotrophs=46,58,3
4.3.5 Nitrogen input manipulation experiment=49,61,2
5. Discussion=51,63,1
5.1 Methane Oxidation=51,63,1
5.1.1 in situ oxidation=51,63,3
5.1.2 Extrapolation to whole Korea=54,66,2
5.1.3 Methane oxidation potential=55,67,1
5.2 Community Structure of Methanotrophic Bacteria=56,68,2
5.3 Relationship of Activities and Community Structure=57,69,1
6. Conclusions=58,70,2
7. References=60,72,13
[abstract in korean]=73,85,2
[acknowledgement]=75,87,2
Table1. The sources and sinks of atmospheric methane(Tg yr-1; IPCC, 2001)(이미지참조)=4,16,1
Table2. Methane oxidation rates in previous reports=6,18,1
Table3. The pH ranges reported methane oxidation=9,21,1
Table4. Previous studies on the inhibitory effect of N addition on methane oxidation rates=11,23,1
Table5. Previous studies about effect factors methane oxidation rates=12,24,1
Table6. Characteristics of type I and type II(Richard and Thomas, 1996)=15,27,1
Table7. Soil chemical properties of site 1, 2 and 3=18,30,1
Table8. Seasonal soil characteristics from May, 2004 to September, 2005=27,39,1
Table9. Correlation matrix for soil characteristics=28,40,1
Table10. Soil characteristics of site 1, 2 and 3=29,41,1
Table11. Correlation for methane oxidation rates and environmental factors=37,49,1
Table12. Shannon diversity index of type I methanotrophs=42,54,1
Table13. Shannon diversity index of type II methanotrophs=45,57,1
Table14. Diversity index of type I and type II methanotrophs of nitrogen input manipulation=50,62,1
Figure1. The range of oxidation rates in forest soil=7,19,1
Figure2. Pathway of methane oxidation(Richard and Thomas, 1996)=14,26,1
Figure3. RuMP pathway(Richard and Thomas, 1996)=16,28,1
Figure4. Serine pathway(Richard and Thomas, 1996)=16,28,1
Figure5. The locations of study site=17,29,1
Figure6. Chamber for in situ measurement=21,33,1
Figure7. Inorganic nitrogen addition experiment=23,35,1
Figure8. in situ methane oxidation rates in Mt. Jumbong=30,42,1
Figure9. Correlation between methane oxidation rates and nitrate concentration in soils=31,43,1
Figure10. Correlation between methane oxidation rates and temperature=32,44,1
Figure11. Potential methane oxidation potential rates in Mt. Jumbong=33,45,1
Figure12. Correlation between methane oxidation potential rates and temperature=34,46,1
Figure13. Correlation between methane oxidation potential rates and DOC=35,47,1
Figure14. Monthly graph of in situ rates and potential rates(Square means the months of discord with in situ and potential oxidation rates)=36,48,1
Figure15. Correlation for in situ oxidation rates and potential rates=36,48,1
Figure16. The results of nitrogen input manipulation experiment=38,50,1
Figure17. Correlation between methane oxidation rate and the amount of nitrogen added=39,51,1
Figure18. The results of methanotrophs specific PCR amplification=40,52,1
Figure19. The change of dominant species of type I methanotrophs=41,53,1
Figure20. Similarity of community structure of type I methanotrophs=42,54,1
Figure21. The change of dominant species of type II methanotrophs=43,55,1
Figure22. Similarity of community structure of type II methanotrophs=44,56,1
Figure23. The results of Principle Component Analysis=44,56,1
Figure24. The relation with methane oxidation(bar)rates and community structure(A,B,C and D:clustered group of community structure)=47,59,1
Figure25. Methane oxidation and diversity index of type I(triangle), type II(circle)methanotrcphs=47,59,1
Figure26. Correlation analysis for oxidation rates and type II methanotrophs=48,60,1
Figure27. Correlation of methane oxidation and diversity index of methanotrophs=48,60,1
Figure28. The similarity analysis for type I methanotrophs community structure=49,61,1
Figure29. The similarity analysis for type II methanotrophs community structure=50,62,1
Figure30. Box plot of methane oxidation rates determined in various forest soils in the world=51,63,1