표제지
목차
I. 緖論 10
II. 實驗材料 및 方法 13
1. 試藥 13
2. InulaseII生成菌의 分離 및 選別 13
3. 使用菌株 및 種培養 14
4. 培地 및 培養方法 14
5. 粗酵素液의 調製 15
6. 培養液으로부터 DFAIII 分離 15
7. 酵素活性 測定 16
8. 分析方法 16
III. 結果 및 考察 18
1. Paper chromatography 法에 依한 DFAIII생성효모 分離 및 選別 18
2. Active carbon column chromatogrphy 및 HLPC 法에 依한 DFAIII의 分離 19
3. DFAIII生成을 爲한 最適反應條件 21
3-1. Inulin 濃度의 影響 21
3-2. 酵素濃度의 影響 22
3-3. 反應時間의 影響 23
3-4. 反應 pH의 影響 24
3-5. 反應溫度의 影響 25
4. DFAIII 生成을 爲한 培地造成 및 最適培養條件 26
4-1. Sucrose 濃度의 影響 26
4-2. YNB 濃度의 影響 27
4-3. Initial pH 의 影響 28
4-4. 培養溫度의 影響 29
4-5. 培地量의 影嚮 30
4-6. 培養時間의 影響 31
5. 牛蒡根 抽出物의 天然培地 및 合成培地에서의 DFAIII 生成 32
5-1. Inulin-YNB 合成培地를 利用한 DFAIII의 生成 32
5-2. Sucrose-YNB 合成培地를 利用한 DFAIII의 生成 33
5-3. 牛蒡根 抽出物의 天然培地를 利用한 DFAIII의 生成 34
IV. 摘要 35
參考文獻 36
抄錄 41
Abstract 42
〈Table 1〉 Seperation and yield of DFAIII in culture broth and eluate 19
〈Table 2〉 Effect of various concentration of inulin on DFAIII production 22
〈Table 3〉 Effect of enzyme concentration on DFAIII production 22
〈Table 4〉 Effect of reaction time on DFAIII production 23
〈Table 5〉 Effect of reaction pH on DFAIII production 24
〈Table 6〉 Effect of reaction temperature on DFAIII production 25
〈Table 7〉 Time course of production of inulaseII and DFAIII in inulin-YNB medium 32
〈Table 8〉 Time course of production of inulaseII and DFAIII in Sucrose-YNB medium 33
〈Table 9〉 Time course of production of inulaseII and DFAIII in Burdock root medium 34
〈Fig. 1〉 Produce mechanism of DFAIII from inulin by the inulin fructotransferase... 12
〈Fig. 2〉 Paper chromatograms of the reaction products produced by the... 18
〈Fig. 3〉 HPLC chromatogram of the culture broth incubated for 24hrs at... 20
〈Fig. 4〉 Paper chromatogram of DFAIII produced from various concentration... 21
〈Fig. 5〉 Paper chromatogram of DFAIII produced from inulin at various... 23
〈Fig. 6〉 Paper chromatogram showing the production of DFAIII in culture... 26
〈Fig. 7〉 Paper chromatogram showing the production of DFAIII in culture... 27
〈Fig. 8〉 Paper chromatogram showing the production of DFAIII in culture... 28
〈Fig. 9〉 Paper chromatogram showing the production of DFAIII in... 29
〈Fig. 10〉 Paper chromatogram showing the production of DFAIII in... 30
〈Fig. 11〉 Paper chromatogram showing the production of DFAIII in... 31