표제지
목차
국문요약 5
Ⅰ.서론 7
Ⅱ. 재료 및 방법 11
1. 실험재료 및 total RNA 분리 11
2. GeneFishing : ACP를 이용한 RT -PCR 11
3. DEGs의 cloning 및 sequencing 12
4. Semi-quantitative RT-PCR 13
5. in situ hybridization 14
6. LCM 과정과 Real-Time PCR 16
7. 통계 분석 17
Ⅲ. 결과 18
1. ACP를 통한 DEG 발굴 결과 18
2. Day 1-DEG의 발현 19
3. Day 5-DEG의 발현 19
4. in situ hybridization 관찰 20
5. Real-Time PCR 분석 20
Ⅳ. 고찰 22
Ⅴ. 결론 26
참고문헌 41
영문요약 45
Table 1 Primer sequences and RT-PCR conditions for DEGs 27
Table 2. Functional fraction of DEGs between the day 1 and day 5 ovaries 28
Figure 1 Structure of ACP (Annealing Control Primer) 29
Figure 2 Typical expression pattern of DEG by Gene Fishing PCR betw een day 1 and day 5 mous e ovary 31
Figure 3. Confirmation of the differential gene expression by semi- quantitative
RT - PCR 33
Figure 4. Localized expression of DEGs (Day 1] Day 5) in day 5 and 4
week- old ovary 35
Figure 5. Localized expression of DEGs (Day 1[ Day 5) in day 5 and 4
week- old ovary 37
Figure 6. Confirmation of the differential gene expression by using Real-Time
PCR 39