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Abstract 8
I. 서언 10
II. 연구사 12
III. 재료 및 방법 17
1. 공시재료 17
2. 생육조사 17
1) 폿트실험 17
2) 한천배지실험 18
3. 식물체내 phenanthrene 축적량 분석 20
4. 통계분석 20
IV. 결과 및 고찰 21
1. 생육조사 21
1) 폿트실험 21
2) 한천배지실험 31
2. 식물체내 phenanthrene 축적량 분석 38
V. 적요 41
인용문헌 43
Table 1. Composition of Murashige & Skoog medium 19
Table 2. Analysis of variance for the effect of PAHs treatment on plant height of soybean plant 23
Table 3. Analysis of variance for the effect of PAHs treatment on plant height of rice plant 24
Table 4. Analysis of variance for the effect of PAHs treatment on chlorophyll fluorescence of soybean plant 25
Table 5. Analysis of variance for the effect of PAHs treatment on chlorophyll fluorescence of rice plant 26
Table 6. Analysis of variance for the effect of PAHs treatment on chlorophyll content (SPAD value)of soybean plant 27
Table 7. Analysis of variance for the effect of PAHs treatment on chlorophyll content (SPAD value)of rice plant 28
Fig. 1. Chemical structure of major PAHs 14
Fig. 2. Influences of phenanthrene on plant height of soybean plant at 21 days after treatment. 23
Fig. 3. Influences of phenanthrene on plant height of rice plant at 21 days after treatment. 24
Fig. 4. Influences of phenanthrene on chlorophyll fluorescence of soybean plant at 21 days after treatment. 25
Fig. 5. Influences of phenanthrene on chlorophyll fluorescence of rice plant at 21 days after treatment. 26
Fig. 6. Influences of phenanthrene on leaf chlorophyll content (SPAD value) of soybean plant at 21 days after treatment. 27
Fig. 7. Influences of phenanthrene on leaf chlorophyll content (SPAD value)of rice plant at 21 days after treatment. 28
Fig. 8. Influences of phenanthrene on dry weight of soybean plant (upper) and rice plant (lower)at 65 days after treatment. 29
Fig. 9. Influences of phenanthrene on dry weight of soybean plant (upper) and rice plant (lower)at 65 days after treatment. 30
Fig. 10. Influences of phenanthrene on plant height of soybean plant same letters are not significantly different at 0.05 probability. 32
Fig. 11. Influences of phenanthrene on fresh weight of soybean plant same letters are not significantly different at 0.05 probability. 33
Fig. 12. Influences of phenanthrene on plant height of rice plantsame letters are not significantly different at 0.05 probability. 34
Fig. 13. Influences of phenanthrene on fresh weight of rice plant same letters are not significantly different at 0.05 probability. 35
Fig. 14. Influences of phenanthrene on chlorophyll fluorescence of soybean plantsame letters are not significantly different at 0.05 probability. 36
Fig. 15. Influences of phenanthrene on chlorophyll fluorescence of rice plantsame letters are not significantly different at 0.05 probability. 37
Fig. 16. Concentration of phenanthrene accumulated in soybean plant. 40
Fig17.Concentration of phenanthrene accumulated in rice plant. 40
초록보기 더보기
Polycyclic aromatic hydrocarbons (PAHs) are a group of ubiquitous hazardous pollutants derived from fossil fuel, various combustion sources and pyrolysis of a wide range of plastics. Because PAHs can be uptake into crop plants, the inhibitory effects on rice and soybean plants were examined in greenhouse and growth chamber experiment. Soil-applied PAHs (phenanthrene of 0, 10, 30, 100 ppm) slightly reduced the plant height and dry weight both in transplanted rice and soybean plant. The inhibitory effect on growth was greater in soybean than rice. Plant height of soybean plants treated by 100 ppm was 58.9 cm and this value was 87.2% of untreated plant. In rice plant, the plant height was less inhibited (96.0% of untreated plant) by 100 ppm at 80 days after treatment (DAT). However, leaf chlorophyll content and chlorophyll fluorescence were less inhibited by PAHs at late growth stage (after heading) although the photosynthesis-related parameters were slightly inhibited from 20 DAT to 70 DAT. In agar medium experiment with five-day old seedlings, inhibition of seedling length and fresh weight at 100 ppm phenanthrene were greater as compared to the experiment with adult plant in pot. Seedling length and fresh weight were reduced by 54.2% and 33.3% for rice and 27.9% and 13.2% for soybean, respectively. The results reflected that PAHs were more inhibitory during juvenile stage than adult stage and more inhibitory to rice plant than soybean for juvenile stage.
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