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Title Page
감사의 글
Contents
Abstract 9
1. Introduction 12
2. Materials and methods 15
2.1. Plant materials 15
2.2. Isolation and purification of an antibacterial compound 15
2.3. Instrumentation 17
2.4. Antibacterial assay 17
2.4.1. Test microorganisms and cultures 17
2.4.2. Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) 18
2.4.3. Time-kill curves 18
2.5. Antioxidant activity 19
2.5.1. Culture of keratinocyte and cell viability 19
2.5.2. Preparation of Propionibacterium acnes 20
2.5.3. DPPH radical scavenging activity 20
2.5.4. Scavenging effect of superoxide anion 21
2.5.5. Scavenging effect of hydrogen peroxide 22
2.5.6. Scavenging effect of nitric oxide radical 22
2.5.7. DCFH-DA Oxidation 23
2.6. Statistics 23
3. Results and discussion 24
3.1. Identification of an antibacterial compound from Baeckea frutescens L. 24
3.2. Antiacne activity 24
3.2.1. Antibacterial activity against Propionibacterim acnes 24
3.2.2. Time-kill curve of Baeckea frutescens L. hexane extract and JS-1 against Propionibacterium acnes 31
3.2.3. Antibacterial spectrum of Baeckea frutescense L. hexane extract and JS-1 33
3.3. Anti-oxidative activity 33
3.3.1. DPPH radical scavenging activity 33
3.3.2. Superoxide anion scavenging activity 36
3.3.3. Hydrogen peroxide scavenging activity 39
3.3.4. Nitric oxide scavenging activity 39
3.3.5. Effect on the P. acnes-induced production of intracellular ROS 41
4. Conclusions 45
5. References 46
국문 요약 50
Table 1. 13C-NMR and ¹H-NMR spectrum data of compound II-III-I(이미지참조) 28
Table 2. Comparison of MIC and MBC of Baeckea frutescense L. hexane extract, JS-1 and commercial antibacterial agents against Propionibacterium acnes 32
Table 3. MIC and MBC of Baeckea frutescens L. hexane extract and JS-1 against skin microorganisms 35
Fig. 1. Synopsis of factors involved in the pathogenesis of acne (Jappe 2003) 13
Fig. 2. Isolation of an antibacterial compound from Baeckea frutescens L. 16
Fig. 3. EI-MASS spectrum of compound II-III-I. 25
Fig. 4. 13C-NMR spectrum of compound II-III-I.(이미지참조) 26
Fig. 5. ¹H-NMR spectrum of compound II-III-I. 27
Fig. 6. 13C-DEPT spectrum of compound II-III-I.(이미지참조) 29
Fig. 7. Chemical structure of JS-1. 30
Fig. 8. Time-kill Curve of (A) Baeckea frutescens L. hexane extract and (B) JS-1 against P. acnes 34
Fig. 9. DPPH radical scavenging effect of Baeckea frutescens L. hexane extract and JS-1 37
Fig. 10. The effects of Baeckea frutescens L. hexane extract and JS-1 on the production of superoxide anion 38
Fig. 11. The effects of Baeckea frutescens L. hexane extract and JS-1 on the production of hydrogen peroxide 40
Fig. 12. The effects of Baeckea frutescens L. hexane extract and JS-1 on the production of nitric oxide 42
Fig. 13. Effects of Baeckea frutescens L. hexane extract and JS-1 on the cell viability 43
Fig. 14. The effects of Baeckea frutescens L. hexane extract and JS-1 on the production of intracellular ROS 44
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